Regulation and crystallization of phosphorylated and dephosphorylated forms of truncated dimeric phenylalanine hydroxylase

Protein Science : a Publication of the Protein Society
B KobeB E Kemp

Abstract

Phenylalanine hydroxylase is regulated in a complex manner, including activation by phosphorylation. It is normally found as an equilibrium of dimeric and tetrameric species, with the tetramer thought to be the active form. We converted the protein to the dimeric form by deleting the C-terminal 24 residues and show that the truncated protein remains active and regulated by phosphorylation. This indicates that changes in the tetrameric quaternary structure of phenylalanine hydroxylase are not required for enzyme activation. Truncation also facilitates crystallization of both phosphorylated and dephosphorylated forms of the enzyme.

References

Oct 24, 1975·Brain Research·M J BrownsteinJ S Kizer
Mar 5, 1991·Journal of Molecular Biology·D BarfordL N Johnson
Apr 5, 1991·Journal of Molecular Biology·R CelikelN H Xuong
Aug 31, 1990·Science·J H HurleyR M Stroud
Aug 24, 1989·Nature·D Barford, L N Johnson
Feb 11, 1970·Biochimica Et Biophysica Acta·R Shinohara, I Ishiguro
Jun 1, 1982·European Journal of Biochemistry·H Nakata, H Fujisawa
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F ChiappelliG Popják
Nov 24, 1981·Biochemistry·R A LazarusS J Benkovic
Aug 7, 1980·Biochimica Et Biophysica Acta·H Nakata, H Fujisawa
Oct 15, 1995·The Biochemical Journal·S E HuftonR G Cotton
Oct 1, 1994·Nature Structural Biology·V L Rath, R J Fletterick
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·D KowlessurS Kaufman
Jan 1, 1994·Annual Review of Genetics·C R ScriverS Kaufman
Jan 14, 1994·Biochemical and Biophysical Research Communications·B A CitronS Kaufman
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·L N Johnson, D Barford
Jun 17, 1993·Nature·H L De BondtS H Kim
Apr 18, 1996·Nature·J HeierhorstB E Kemp
Aug 1, 1996·Nature Structural Biology·A A RussoN P Pavletich
Jan 1, 1991·Neurochemistry International·X Liu, K E Vrana

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Citations

Jul 19, 2000·Journal of Neuroscience Research·G J YohrlingK E Vrana
Jul 31, 1998·Protein Science : a Publication of the Protein Society·R J CenterP Poumbourios
Mar 1, 2003·Insect Biochemistry and Molecular Biology·J K JohnsonB M Christensen
Dec 4, 2003·Biochemistry·Paul F Fitzpatrick
May 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Peter N DoddsJeffrey G Ellis
Jul 27, 2002·Protein Science : a Publication of the Protein Society·James HorneBostjan Kobe
Jul 9, 2009·Physiological Reviews·Gregory R Steinberg, Bruce E Kemp
Jun 27, 1998·The Journal of Biological Chemistry·F FusettiR C Stevens
Feb 19, 2002·Chemical Reviews·Torgeir Flatmark, Raymond C. Stevens
Dec 24, 2010·Archives of Biochemistry and Biophysics·S Colette DaubnerShanzhi Wang

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