PMID: 8944685Nov 1, 1996Paper

Regulation and function of extracellular matrix intestinal epithelial restitution in vitro

The American Journal of Physiology
M GökeDaniel K Podolsky

Abstract

Repair of epithelial injury in the gastrointestinal tract is initially accomplished by migration of epithelial cells from the wound edge ("restitution"). To assess expression and function of the extracellular matrix (ECM) in the restitution phase after epithelial injury, in vitro studies using wounded monolayers or a rat intestinal epithelium-derived cell line (IEC-6) were undertaken. IEC-6 cells expressed fibronectin (FN) mRNA and protein in large amounts and lesser quantities of laminin-beta 1 (LN beta 1) and LN gamma 1. Collagen IV (Col IV) was weakly expressed, and LN alpha 1 was not detected. After wounding a significant decrease in FN, LN beta 1, LN gamma 1, and Col IV alpha 1 mRNA steady-state levels was observed; mean content 24 h after wounding was reduced by 75-90%. FN, LN, and Col IV proteins were also reduced. The downregulation of these ECM transcripts and proteins could be substantially prevented by transforming growth factor-beta 1, a restitution-promoting growth factor. In addition to changes of expression, the distribution of FN and LN was also altered in migrating cells after wounding, as assessed by immunofluorescence. Arg-Gly-Asp peptides that recognize the major cell attachment site on FN and antibodies rec...Continue Reading

Citations

Mar 18, 2005·JPEN. Journal of Parenteral and Enteral Nutrition·Oded ZmoraAmram Ayalon
Aug 10, 2007·American Journal of Physiology. Cell Physiology·Silvina GrassoSilvia Chifflet
Apr 11, 2008·American Journal of Physiology. Cell Physiology·Lakshmi S ChaturvediMarc D Basson
Dec 18, 2001·American Journal of Physiology. Cell Physiology·B C McKaigY R Mahida
Feb 8, 2003·The Journal of Biological Chemistry·Ramesh M RayLeonard R Johnson
Apr 13, 2007·Physiological Reviews·Anthony T BlikslagerJack Odle
Dec 2, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dinithi PeirisR John MacLeod
Sep 19, 2012·Journal of Signal Transduction·Yannick D BenoitJean-François Beaulieu
Oct 22, 2004·American Journal of Physiology. Cell Physiology·Rajiv J VaidyaLeonard R Johnson
Nov 11, 2011·Journal of Biomaterials Applications·Zhanhui WangShaoxiang Zhang
Apr 25, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Deborah A Swartz-BasileMarc S Levin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.