PMID: 2109668Jan 1, 1990Paper

Regulation and rate of the hexose monophosphate shunt in Rana ridibunda erythrocytes

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
M Kaloyianni, M Kalomenopoulou

Abstract

1. Resting rates of Rana ridibunda erythrocyte glucose consumption and 14CO2 production from 1-14C-glucose were found to be significantly lower than the respective values in human erythrocytes. 2. In the presence of 1-14C-glucose Methylene Blue stimulated 14CO2 production 7-fold, while in the presence of 6-14C-glucose Methylene Blue stimulated 14CO2 production 1.2-fold. 3. The Km of G-6-PD for G-6-P and NADP were 29 and 12 microM, respectively while the Km of 6-PGD for 6-PG and NADP were 83 and 32 microM, respectively. The Ki of G-6-PD and 6-PGD for NADPH were 80 and 12 microM, respectively. 4. Excess amounts of NADP resulted in a significant decrease of 14CO2 production from 1-14C-glucose in total haemolysates. 5. ATP, ADP and fructose diphosphate inhibited both G-6-PD and 6-PGD, the latter being more sensitive than G-6-PD to their inhibitory effect, 2,3-DPG and reduced and oxidized glutathione showed a marked inhibitory effect on 6-PGD, while the phosphorylated trioses inhibited only G-6-PD. 6. Physiological concentrations of oxidized glutathione decreased the inhibition exercised by NADPH on G-6-PD. 7. The possible role of the two dehydrogenases in the regulation of the HMS is discussed.

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Citations

Jul 1, 1994·Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology·M Kaloyianni, K Moutou
Mar 1, 1994·Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology·M KaloyianniE G Gounaris
Jan 7, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M de C CesarR Rosa
Nov 3, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·T AlekhovaC Dournon
May 2, 2002·Physiologia Plantarum·Tahei KawachiYoshikiyo Oji

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