Abstract
Heterotrimeric G-proteins, as well as small GTPases of the Rho and ADP-ribosylation factor (ARF) family, are implicated in the regulation of lipid kinases, including PtdIns 4-kinases and PtdIns(4)P 5-kinases. Here, we describe a PtdIns 4-kinase activity on immature secretory granules (ISGs), regulated secretory organelles formed from the trans-Golgi network (TGN), and investigate the regulation of PtdIns4P levels on these membranes. Over 50% of the PtdIns 4-kinase activity on ISGs is inhibited by both a low concentration of adenosine and the monoclonal antibody 4C5G, a specific inhibitor of the type II PtdIns 4-kinase. Treatment of ISGs with mastoparan 7 (M7) stimulates the type II PtdIns 4-kinase via pertussis-toxin-sensitive G(i)/G(0) proteins, which, in contrast with previous results obtained with chromaffin granules [Gasman, Chasserot-Golaz, Hubert, Aunis and Bader (1998) J. Biol. Chem. 273, 16913-16920], does not require Rho A, B or C. M7 treatment also leads to an inhibition in the recruitment of ARF to ISG membranes: this inhibition is not dependent on G(i)/G(0) activation, and is not linked to the stimulation of PtdIns 4-kinase observed with M7. PtdIns 4-kinase activity on ISGs is not regulated by myristoylated ARF1-GTP...Continue Reading
References
Dec 1, 1992·The Journal of Cell Biology·R SchwaningerW E Balch
Mar 26, 1992·Nature·N T KtistakisM G Roth
Mar 9, 1990·Cell·S A Tooze, W B Huttner
May 16, 1985·Nature·M WhitmanT M Roberts
Dec 1, 1994·The Journal of Cell Biology·P J Weidman, W M Winter
Feb 1, 1996·The Journal of Cell Biology·A S DittieS A Tooze
Apr 8, 1996·FEBS Letters·F A Barr, W B Huttner
Jun 27, 1998·The Journal of Biological Chemistry·S GasmanM F Bader
Oct 6, 1998·Annual Review of Biochemistry·D A FrumanL C Cantley
Oct 28, 1998·Cell·J Goldberg
Nov 13, 1999·Nature Cell Biology·A GodiM A De Matteis
Mar 11, 2000·Molecular Biology of the Cell·A MayerA Haas
Apr 5, 2000·The Journal of Biological Chemistry·D H JonesS Cockcroft
May 16, 2000·The Journal of Biological Chemistry·C AustinS A Tooze
Jun 24, 2000·Biochimie·V A Klenchin, T F Martin
Feb 13, 2001·European Journal of Biochemistry·Y HoriT Asakura
Mar 13, 2001·The Journal of Biological Chemistry·B BarylkoJ P Albanesi
Mar 30, 2001·The Journal of Biological Chemistry·S MinogueJ J Hsuan
Apr 18, 2001·Trends in Cell Biology·S A ToozeW B Huttner
Jun 16, 2001·Molecular Biology of the Cell·F WendlerS A Tooze
Jul 17, 2001·Current Opinion in Cell Biology·T F Martin
Oct 18, 2001·Neuron·S L OsborneG Schiavo
Citations
Oct 12, 2012·Molecular Neurobiology·Emma L ClaytonMark G Waugh
Jun 23, 2007·Journal of Proteome Research·Michael J RindlerThomas A Neubert
Jun 2, 2004·Nature Cell Biology·Maria Antonietta De Matteis, Anna Godi
Oct 10, 2008·Molecular Biology of the Cell·Peter J WenFrederic A Meunier
Aug 31, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Hélène LemieuxCharles L Hoppel
Jul 14, 2012·Development·Jason BurgessJulie A Brill
Aug 14, 2003·Cell·Ying Jie WangHelen L Yin