Regulation and translocation of ATP-dependent apical membrane proteins in rat liver

The American Journal of Physiology
Z GatmaitanIrwin M Arias

Abstract

The bile canalicular membrane contains four specific ATP-dependent transport processes that are involved in secretion of bile acids, non-bile acid organic anions (mrp1), phospholipids (mdr2), and organic cations (mdr3). The aim of this study was to determine how the canalicular presence of these transport proteins is regulated. Canalicular membrane vesicles (CMV) were prepared from livers of rats treated with taurocholate (TC) and/or dibutyryl-adenosine 3',5'-cycle monophosphate (DBcAMP) with and without pretreatment with colchicine. Transport studies were performed with radiolabeled substrates. Changes in the relative amounts of transport proteins were determined by Western blots. Compared with controls, the specific activity of each of the transport processes was enhanced 1.5- and 3-fold with TC and DBcAMP treatments, respectively. Western blots revealed the same increases with mdr2 and mdr3. Pretreatment of rats with colchicine prevented these responses fully with TC treatment, whereas only partial prevention was obtained with DBcAMP treatment. Besides the ATP-dependent transporters, the relative specific activities of the canalicular membrane ectoenzyme markers, leucine aminopeptidase and gamma-glutamyltranspeptidase, were ...Continue Reading

Citations

Feb 5, 2002·Annual Review of Physiology·Peter J Meier, B Stieger
Mar 6, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christopher M SchonhoffM Sawkat Anwer
Mar 29, 2003·Physiological Reviews·Michael Trauner, James L Boyer
Mar 26, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christopher M SchonhoffM Sawkat Anwer
Feb 5, 2002·Annual Review of Physiology·Helmut Kipp, Irwin M Arias
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Peter J. Meier, Bruno Stieger
Jan 15, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Priyamvada ChandraKim L R Brouwer
Jul 7, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Laura FouassierChantal Housset
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Tatehiro KagawaIrwin M Arias
Apr 19, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Suniti MisraIrwin M Arias
Oct 20, 2006·Pflügers Archiv : European journal of physiology·Bruno StiegerPeter J Meier
May 23, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yasuhiro YamazakiJunko Sugatani
Apr 19, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Fernando A CrocenziMarcelo G Roma
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Luca MarucciGene LeSage
Oct 7, 2008·World Journal of Gastroenterology : WJG·Alejandro Esteller
Jan 17, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Allan W Wolkoff, David E Cohen

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