Regulation Between HSF1 Isoforms and HSPs Contributes to the Variation in Thermal Tolerance Between Two Oyster Congeners

Frontiers in Genetics
Youli LiuGuofan Zhang

Abstract

Heat shock transcription factor 1 (HSF1) plays an important role in regulating heat shock, which can activate heat shock proteins (HSPs). HSPs can protect organisms from thermal stress. Oysters in the intertidal zone can tolerate thermal stress. The Pacific oyster (Crassostrea gigas gigas) and Fujian oyster (C. gigas angulata)-allopatric subspecies with distinct thermal tolerances-make good study specimens for analyzing and comparing thermal stress regulation. We cloned and compared HSF1 isoforms, which is highly expressed under heat shock conditions in the two subspecies. The results revealed that two isoforms (HSF1a and HSF1d) respond to heat shock in both Pacific and Fujian oysters, and different heat shock conditions led to various combinations of isoforms. Subcellular localization showed that isoforms gathered in the nucleus when exposed to heat shock. The co-immunoprecipitation revealed that HSF1d can be a dimer. In addition, we selected HSPs that are expressed under the heat shock response, according to the RNA-seq and proteomic analyses. For the HSPs, we analyzed the coding part and the promoter sequences. The result showed that the domains of HSPs are conserved in two subspecies, but the promoters are significantly dif...Continue Reading

References

Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E LamN H Chua
Jan 1, 1995·Annual Review of Cell and Developmental Biology·C Wu
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L PirkkalaL Sistonen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 17, 2002·Cell Stress & Chaperones·Annamaria PianoElena Fabbri
Apr 2, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Shinya Kawabe, Yoshihiro Yokoyama
Jul 13, 2011·Cold Spring Harbor Perspectives in Biology·Bryan ChenJudith Frydman
Oct 29, 2011·Biochimica Et Biophysica Acta·Klaus-Dieter ScharfLutz Nover
Jul 24, 2012·Genome Research·Gil HornungNaama Barkai
Sep 14, 2013·Science·Malin L PinskySimon A Levin
Aug 30, 2014·Cell Stress & Chaperones·Viktoria OrtnerThomas Czerny
Aug 10, 2015·Marine Environmental Research·Adán Valenzuela-CastilloJosé Manuel Grijalva-Chon
Jan 20, 2016·Molecular Biology and Evolution·Brian P H MetzgerPatricia J Wittkopp
Jun 25, 2016·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Hyun-Jeong LimJae-Sung Rhee
Aug 31, 2017·Nature Reviews. Molecular Cell Biology·Rocio Gomez-PastorDennis J Thiele
Dec 21, 2017·Proceedings of the National Academy of Sciences of the United States of America·Fabien DuveauPatricia J Wittkopp
Jan 17, 2019·Developmental and Comparative Immunology·Huan ZhangLinsheng Song

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Datasets Mentioned

BETA
MT737786
MT737796

Methods Mentioned

BETA
electrophoresis
PCR
confocal microscopy
transfection
Assay
co-immunoprecipitation
pulldown

Software Mentioned

bio editing
QMEAN
clustal omega
MODEL
SWISS

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