Regulation by CRAMP of the responses of murine peritoneal macrophages to extracellular ATP

Biochimica Et Biophysica Acta
Michèle SeilJean-Paul Dehaye

Abstract

Peritoneal macrophages were isolated from wild type (WT) mice and from mice invalidated for the P2X(7) receptor (KO) which had been pretreated with thioglycolate. In cells from WT mice, 1 mM ATP increased the intracellular concentration of calcium ([Ca(2+)](i)), the uptake of ethidium bromide, the production of reactive oxygen species (ROS), the secretion of IL-1beta, the release of oleic acid and of lactate dehydrogenase; it decreased the intracellular concentration of potassium ([K(+)](i)). In KO mice, ATP transiently increased the [Ca(2+)](i) confirming that the P2X(7) receptor is a major receptor of peritoneal macrophages. WKYMVm, an agonist of receptors for formylated peptides (FPR) also increased the [Ca(2+)](i) in murine macrophages. The slight increase of the [Ca(2+)](i) was strongly potentiated by ivermectin confirming the expression of functional P2X(4) receptors by murine peritoneal macrophages. CRAMP, the unique antimicrobial peptide derived from cathelin in mouse inhibited all the responses coupled to P2X(7) receptors in macrophages from WT mice. Agonists for FPR had no effect on the increase of the [Ca(2+)](i) in response to ATP. CRAMP had no effect on the increase of the [Ca(2+)](i) evoked by a combination of ATP...Continue Reading

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Citations

Jul 23, 2014·Odontology·Yuko NakamichiNobuyuki Udagawa
Dec 19, 2012·Cellular Immunology·Dieter VandammeLiliane Schoofs
Jun 29, 2011·Innate Immunity·Michèle SeilJean-Paul Dehaye
Oct 25, 2016·Peptides·Eddy-Tim VerjansLiliane Schoofs
Nov 3, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robson FariaLuiz Alves
Jun 15, 2014·Pharmacological Reviews·Rachael BartlettRonald Sluyter

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