Regulation by thyroid hormone of the expression of basement membrane components in rat prepubertal Sertoli cells

Endocrinology
Salvatore UlisseE A Jannini

Abstract

The present study reports the modulation of basement membrane (BM) components, laminin, entactin, and type IV collagen, expression in prepubertal rat Sertoli cell by the thyroid hormone T3. Immunocytochemical studies of permeabilized Sertoli cells in culture showed that T3 treatment (10[-7] M for 24 h) increased the number of cells staining positive for laminin and/or entactin (from 58 +/- 5.3% to 86.4 +/- 6.5%, P < 0.01). In contrast, a strong inhibition of type IV collagen immunopositivity was observed. Western blot analysis of Sertoli cell-conditioned media indicated that T3 treatment significantly (P < 0.01) increased the level of secreted entactin by 60-65% without affecting the levels of laminin A and B1/B2 chains. Moreover, thyroid hormone treatment of Sertoli cells significantly reduced type IV collagen secretion by 62% (P < 0.05). Slot blot analysis of poly-A RNA demonstrated a significant (P < 0.01) increase in the level of entactin messenger RNA (mRNA) by 140% (P < 0.01) and a 50% reduction of type IV collagen alpha1 chain mRNA after thyroid hormone treatment. No effect of the hormone was observed on the accumulation of the laminin B1 and B2 chain mRNAs in Sertoli cell cultures. These effects cannot be ascribed to ch...Continue Reading

Citations

Aug 30, 2008·The Journal of Endocrinology·Márcia Santos WagnerAna Luiza Maia
Oct 17, 2013·Hormones & Cancer·Vijayakumar GovindarajA J Rao
Nov 22, 2014·Frontiers in Endocrinology·Ying GaoC Yan Cheng
Dec 14, 2007·Free Radical Research·Rodrigo J S DalmolinJosé C F Moreira
Aug 13, 2003·Cell Transplantation·Dwaine F EmerichCraig R Halberstadt
Mar 14, 2002·Molecular Reproduction and Development·Emilce S DiazBerta Denduchis
Sep 28, 2018·Acta Biologica Hungarica·Fatemeh AlipourElnaz Khordad
Jul 29, 2009·Microscopy Research and Technique·Marcia Santos WagnerAna Luiza Maia
Mar 10, 2000·Journal of Endocrinological Investigation·E A JanniniM D'Armiento
Jun 30, 2009·Journal of Cellular Physiology·Cecilia Verga FalzacappaSilvia Misiti

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