PMID: 8605293Feb 1, 1996Paper

Regulation, expression and function of a new basic chitinase gene in rice (Oryza sativa L.)

Plant Molecular Biology
Y XuC Lamb

Abstract

A new basic chitinase gene, designated RC24, was isolated from a rice genomic library. The predicted RC24 protein contains 322 amino acid residues and exhibits 68% to 95% amino acid identity with known class I rice chitinases. RC24 protein expressed in Escherichia coli exhibited chitinase activity and strongly inhibited bacterial growth. Two transcription start sites of the RC24 gene were mapped by primer extension analysis of both rice native RNA and in vitro transcribed RNA using a RC24 promoter/GUS (beta-glucuronidase) gene fusion as a template. The 5'-flanking region of RC24 contained several putative stress-responsive cis-acting elements. A basal level of RC24 transcripts was detected in rice root and stem tissues, but not in leaf tissues. RC24 transcripts rapidly accumulated within 1 h after fungal elicitor treatment of suspension-cultured cells, and the levels continued to increase for at least 9 h. RC24 transcript accumulation was also observed in intact leaf tissues upon wounding, Transgenic rice plants containing the RC24/GUS gene fusion further confirmed that the RC24 gene showed a tissue-specific expression pattern and that transcription of the RC24 propmoter was sensitively and rapidly activated by wounding.

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Citations

Jan 1, 2002·The Arabidopsis Book·Paul A Passarinho, Sacco C de Vries
Sep 3, 2008·Science in China. Series C, Life Sciences·Q YaoM Hong
Sep 21, 2000·Plant Science : an International Journal of Experimental Plant Biology·A K Tyagi, A Mohanty
Oct 11, 2003·Biotechnology Advances·C C Giri, G Vijaya Laxmi
Jul 3, 1998·Current Opinion in Biotechnology·R Cohen-Kupiec, I Chet
Dec 14, 2011·Journal of Experimental Botany·Patrick KabouwArjen Biere
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Masahiro Sugiura
Jul 2, 2003·Bioscience, Biotechnology, and Biochemistry·Nam-Hai TruongYoshifumi Itoh
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·C Y KimM J Cho
Jun 30, 2006·Plant Physiology and Biochemistry : PPB·Nam-Soo JwaRandeep Rakwal
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon

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