Regulation of γ-Aminobutyrate (GABA) Utilization in Corynebacterium glutamicum by the PucR-Type Transcriptional Regulator GabR and by Alternative Nitrogen and Carbon Sources

Frontiers in Microbiology
Lingfeng ZhuMichael Bott

Abstract

γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid mainly formed by decarboxylation of L-glutamate and is widespread in nature from microorganisms to plants and animals. In this study, we analyzed the regulation of GABA utilization by the Gram-positive soil bacterium Corynebacterium glutamicum, which serves as model organism of the phylum Actinobacteria. We show that GABA usage is subject to both specific and global regulatory mechanisms. Transcriptomics revealed that the gabTDP genes encoding GABA transaminase, succinate semialdehyde dehydrogenase, and GABA permease, respectively, were highly induced in GABA-grown cells compared to glucose-grown cells. Expression of the gabTDP genes was dependent on GABA and the PucR-type transcriptional regulator GabR, which is encoded divergently to gabT. A ΔgabR mutant failed to grow with GABA, but not with glucose. Growth of the mutant on GABA was restored by plasmid-based expression of gabR or of gabTDP, indicating that no further genes are specifically required for GABA utilization. Purified GabR (calculated mass 55.75 kDa) formed an octamer with an apparent mass of 420 kDa and bound to two inverted repeats in the gabR-gabT intergenic region. Glucose, gluconate, and myo-inosito...Continue Reading

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Citations

May 12, 2021·Microbial Cell Factories·Carina PrellVolker F Wendisch

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Datasets Mentioned

BETA
GSE138829
GSE156688

Methods Mentioned

BETA
PCR
RNAseq
gel filtration
Protein Assay
reverse phase chromatography
size exclusion chromatography
deamination

Software Mentioned

Trim Sequences
Sim
CLC Genomics Workbench
GenePix Pro
BioConductor R packages
Per
MultiGeneBlast
limma
ReadXplorer
marray

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