Regulation of 25-hydroxyvitamin D-1alpha-hydroxylase by epidermal growth factor in prostate cells

The Journal of Steroid Biochemistry and Molecular Biology
Lilin WangTai C Chen

Abstract

Accumulating data suggest that local production of 1alpha,25-dihydroxyvitamin D (1alpha,25(OH)(2)D) could provide an important cell growth regulatory mechanism in an autocrine fashion in prostate cells. Previously, we demonstrated a differential expression of 1alpha-OHase enzymatic activity among noncancerous (PZHPV-7) and cancer cells (PC-3, DU145, LNCaP), which appears to correlate with 1alpha-OHase m-RNA synthesis and its promoter activities. Since it is well-established that EGF regulates the proliferation of prostate cells via autocrine and paracrine loops and 1alpha,25(OH)(2)D inhibites prostate cell proliferation, we investigated if EGF also regulated 1alpha-OHase expression in prostate cells. We found that EGF upregulated 1alpha-OHase promoter activity and enzyme activity in PZ-HPV-7 and that 1alpha,25(OH)(2)D(3) inhibited EGF-dependent up-regulation of 1alpha-OHase enzymatic activity. Moreover, the EGF-stimulated promoter activity was inhibited 70% by the MAPKK inhibitor, PD98059, suggesting that the MAPK pathway may be one pathway involved in the regulation of prostatic 1alpha-OHase by EGF to increase1alpha,25(OH)(2)D synthesis as a feedback regulator of cell growth. Because EGF has no effect on 1alpha-OHase promoter ...Continue Reading

References

Jan 1, 1987·Annual Review of Biochemistry·G Carpenter
Oct 28, 1998·Physiological Reviews·G JonesH F DeLuca
Aug 24, 1999·Cancer Metastasis Reviews·G J Miller
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·B Mayr, M Montminy
Jun 11, 2002·Annual Review of Nutrition·John L OmdahlBrian K May
Jul 26, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Lyman W WhitlatchTai C Chen
Mar 22, 2003·Experimental Cell Research·H Steven Wiley
Aug 6, 2003·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Lilin WangTai C Chen
Feb 5, 2011·Current Topics in Medicinal Chemistry·G Chiellini, Hector F DeLuca
Oct 26, 2012·Anti-cancer Agents in Medicinal Chemistry·Gary G Schwartz

❮ Previous
Next ❯

Citations

Apr 28, 2009·The British Journal of Nutrition·Christian OudshoornEdgar M Colin
Apr 8, 2014·Nature Reviews. Cancer·David FeldmanBrian J Feldman
Dec 5, 2008·Nutrition Reviews·Evelyne van EttenLut Overbergh
Feb 14, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Cynthia S RitterAlex J Brown
Apr 29, 2015·Canadian Journal of Physiology and Pharmacology·Yotaro MatsumotoTai C Chen
Oct 9, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Jia LiDorothy Teegarden
Oct 14, 2016·PloS One·Debora Lucia Seguro DanilovicAna Oliveira Hoff
Oct 9, 2018·Cognition & Emotion·Yan GeJinjing Song

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.