Regulation of 25-hydroxyvitamin D3 1alpha-hydroxylase, 1,25-dihydroxyvitamin D3 24-hydroxylase and vitamin D receptor gene expression by 8-bromo cyclic AMP in cultured human syncytiotrophoblast cells

The Journal of Steroid Biochemistry and Molecular Biology
E AvilaF Larrea

Abstract

In vitro differentiation of human trophoblast cells is a dynamic process accompanied by increasing intracellular levels of cyclic AMP (cAMP). Signaling through cAMP in this tissue is central to hormone expression and cytodifferentiation. In the present study, we analyzed transcriptional regulation of key enzymes involved in vitamin D endocrine system during in vitro syncytiotrophoblast formation. Total RNA was isolated from human trophoblast cells and subjected to reverse transcription, polymerase chain reaction and Southern blot analysis using specific primers and radiolabeled probes. During syncytium formation 25-hydroxyvitamin D(3) 1alpha-hydroxylase (CYP27B1) was decreased while vitamin D receptor (VDR) gene remained unaffected. No 1,25-dihydroxyvitamin D(3) 24-hydroxylase (CYP24) transcription signal was detected. Nevertheless, incubations in the presence of 8-bromo cAMP (1.5mM) resulted in CYP24 induction and CYP27B1 inhibition, respectively. The overall data showed that cultured human syncytiotrophoblasts express key enzymes involved in vitamin D metabolism, as well as VDR. The results support previous findings that human placenta is a source of 1,25-dihydroxyvitamin D(3) (1,25-(OH)(2)D(3)), which synthesis is regulated ...Continue Reading

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Citations

Jul 22, 2010·European Journal of Human Genetics : EJHG·Emilie SundqvistIngrid Kockum
Jan 24, 2008·Reproductive Biology and Endocrinology : RB&E·David BarreraLorenza Díaz
May 19, 2012·Endocrine Reviews·Clifford J RosenChristopher S Kovacs
Dec 5, 2008·Nutrition Reviews·Sam Samuel, Michael D Sitrin
Jan 27, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Euclides AvilaFernando Larrea
Jan 30, 2007·The Journal of Steroid Biochemistry and Molecular Biology·David BarreraLorenza Díaz
Jul 17, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Geoffrey N HendyDavid E C Cole
Jun 3, 2015·Nutrients·Juliana de Castro KronerMario Fabri
Nov 9, 2017·International Journal of Molecular Sciences·Julia KnablUdo Jeschke
Dec 29, 2005·American Journal of Public Health·Cedric F GarlandMichael F Holick
Apr 6, 2006·Journal of the National Cancer Institute·Gary G Schwartz, William J Blot
May 9, 2007·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Faustino R Pérez-López
Feb 27, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·T P H NguyenP Murthi
Jan 9, 2013·International Journal of Clinical Practice·Panagiotis AnagnostisDimitrios G Goulis
Mar 30, 2016·Archives of Gynecology and Obstetrics·Shi Shi LvYong Xu
Mar 2, 2018·American Journal of Reproductive Immunology : AJRI·Andrea Olmos-OrtizLorenza Díaz
Mar 19, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Beth J KirbyChristopher S Kovacs
Oct 29, 2020·Reproductive Sciences·Yang MuJing Yang
Jan 10, 2009·Molecular and Cellular Endocrinology·Katerina PospechovaPetr Pavek

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