Regulation of 5'-adenosine monophosphate deaminase in the freeze tolerant wood frog, Rana sylvatica.

BMC Biochemistry
Christopher A Dieni, Kenneth B Storey

Abstract

The wood frog, Rana sylvatica, is one of a few vertebrate species that have developed natural freeze tolerance, surviving days or weeks with 65-70% of its total body water frozen in extracellular ice masses. Frozen frogs exhibit no vital signs and their organs must endure multiple stresses, particularly long term anoxia and ischemia. Maintenance of cellular energy supply is critical to viability in the frozen state and in skeletal muscle, AMP deaminase (AMPD) plays a key role in stabilizing cellular energetics. The present study investigated AMPD control in wood frog muscle. Wood frog AMPD was subject to multiple regulatory controls: binding to subcellular structures, protein phosphorylation, and effects of allosteric effectors, cryoprotectants and temperature. The percentage of bound AMPD activity increased from 20 to 35% with the transition to the frozen state. Bound AMPD showed altered kinetic parameters compared with the free enzyme (S0.5 AMP was reduced, Hill coefficient fell to approximately 1.0) and the transition to the frozen state led to a 3-fold increase in S0.5 AMP of the bound enzyme. AMPD was a target of protein phosphorylation. Bound AMPD from control frogs proved to be a low phosphate form with a low S0.5 AMP an...Continue Reading

References

Jun 1, 1990·The Quarterly Review of Biology·K B Storey, J M Storey
Apr 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·K B Storey
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J P Raffin
Nov 14, 1997·Biochemistry and Molecular Biology International·V I LushchakK B Storey
Feb 26, 2000·Cardiovascular Research·L A GustafsonK Kroll
Mar 24, 2000·Archives of Biochemistry and Biophysics·T E English, K B Storey
Nov 30, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Marcelo Hermes-Lima, Tania Zenteno-Savín
Aug 9, 2003·Physiological and Biochemical Zoology : PBZ·Jason T IrwinRichard E Lee
May 10, 2007·The Journal of Experimental Biology·Kenneth B Storey, Janet M Storey

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Citations

Jun 10, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Christopher A Dieni, Kenneth B Storey
May 28, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Christopher A Dieni, Kenneth B Storey
Mar 7, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Christopher A Dieni, Kenneth B Storey
Jul 22, 2015·PloS One·Jessica S Veysey Powell, Kimberly J Babbitt
Jul 19, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Oscar A AguilarKenneth B Storey
May 23, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jing ZhangKenneth B Storey
Feb 10, 2017·Physiological Reviews·Kenneth B Storey, Janet M Storey
Jun 12, 2017·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Liam J Hawkins, Kenneth B Storey
Dec 11, 2020·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Khalil Abnous, Kenneth B Storey
Jan 17, 2012·The Journal of Experimental Biology·Christopher A DieniKenneth B Storey

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Methods Mentioned

BETA
environmental stress
deamination
IEC

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