Regulation of a cloned epithelial Na+ channel by its beta- and gamma-subunits

The American Journal of Physiology
M S AwaydaD J Benos

Abstract

Using the Xenopus oocyte expression system, we examined the mechanisms by which the beta- and gamma-subunits of an epithelial Na+ channel (ENaC) regulate alpha-subunit channel activity and the mechanisms by which beta-subunit truncations cause ENaC activation. Expression of alpha-ENaC alone produced small amiloride-sensitive currents (-43 +/- 10 nA, n = 7). These currents increased > 30-fold with the coexpression of beta- and gamma-ENaC to -1,476 +/- 254 nA (n = 20). This increase was accompanied by a 3.1- and 2.7-fold increase of membrane fluorescence intensity in the animal and vegetal poles of the oocyte, respectively, with use of an antibody directed against the alpha-subunit of ENaC. Truncation of the last 75 amino acids of the beta-subunit COOH terminus, as found in the original pedigree of individuals with Liddle's syndrome, caused a 4.4-fold (n = 17) increase of the amiloride-sensitive currents compared with wild-type alpha beta gamma-ENaC. This was accompanied by a 35% increase of animal pole membrane fluorescence intensity. Injection of a 30-amino acid peptide with sequence identity to the COOH terminus of the human beta-ENaC significantly reduced the amiloride-sensitive currents by 40-50%. These observations suggest ...Continue Reading

References

Sep 25, 1984·Nucleic Acids Research·P A Krieg, D A Melton
Nov 1, 1995·Biochimica Et Biophysica Acta·W M WeberW Clauss
Nov 17, 1995·The Journal of Biological Chemistry·R WaldmannM Lazdunski
Jun 1, 1995·The American Journal of Physiology·M S AwaydaD J Benos
Jul 1, 1995·The American Journal of Physiology·A PuotiB C Rossier
May 1, 1995·The American Journal of Physiology·F J McDonaldM J Welsh
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·L SchildB C Rossier
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·N VoilleyP Barbry
Jan 12, 1996·The Journal of Biological Chemistry·I I IsmailovD J Benos
Aug 1, 1996·The American Journal of Physiology·C AsherH Garty
Jul 1, 1996·The Journal of General Physiology·M S AwaydaD J Benos
Jan 1, 1996·The Journal of Membrane Biology·I I IsmailovD J Benos
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·D FirsovB C Rossier
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·N KizerK Hruska

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Citations

Jun 13, 2001·American Journal of Physiology. Cell Physiology·S J CopelandD J Benos
Nov 9, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew B FotiaSharad Kumar
Feb 8, 2006·The Journal of Membrane Biology·M M Reddy, P M Quinton
Oct 6, 1998·The Journal of General Physiology·G K Fyfe, C M Canessa
Mar 13, 2001·The Journal of Biological Chemistry·K F HarveyS Kumar
Jun 8, 1999·The Journal of Biological Chemistry·A Náray-Fejes-TóthG Fejes-Tóth
Aug 5, 2003·The Journal of Biological Chemistry·Rachell E BoothJames D Stockand
Aug 21, 2003·American Journal of Physiology. Renal Physiology·Jakob NielsenSøren Nielsen
Mar 3, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Katja SobczakWolf-Michael Weber
Jun 10, 2004·The Journal of Biological Chemistry·He-Ping MaDouglas C Eaton
Feb 3, 2005·American Journal of Physiology. Renal Physiology·Giovambattista CapassoGiuseppe Bianchi
Apr 13, 2004·American Journal of Physiology. Renal Physiology·Soo Wan KimSøren Nielsen
Jan 27, 2012·American Journal of Physiology. Cell Physiology·Yawar J QadriCatherine M Fuller
Aug 5, 1998·The Journal of General Physiology·M S Awayda, M Subramanyam
Nov 7, 1999·The Journal of Biological Chemistry·M L ChalfantB A Stanton
Sep 25, 2007·Pflügers Archiv : European journal of physiology·Kristina Kusche-VihrogHans Oberleithner
Mar 1, 2006·Electrolyte & Blood Pressure : E & BP·Soo Wan Kim
Mar 1, 2013·Journal of Cell Science·Hsin-Ya YangR Rivkah Isseroff
Nov 26, 1999·The Journal of Investigative Dermatology·Y OdaT Mauro
Nov 22, 2005·Seminars in Nephrology·Giovambattista CapassoMaria Rizzo
Jan 16, 1999·Biochemistry·I I IsmailovD J Benos

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