Regulation of a geminivirus coat protein promoter by AL2 protein (TrAP): evidence for activation and derepression mechanisms

Virology
Garry Sunter, D M Bisaro

Abstract

Tomato golden mosaic virus (TGMV) is a bipartite member of the subgroup III Geminiviridae. Like all geminiviruses, TGMV replicates in the nucleus of susceptible cells by rolling circle replication (RCR). Double-stranded replicative form DNA generated during RCR serves as template for the transcription of viral genes by RNA polymerase II and the associated cellular transcription machinery. Previous studies in tobacco protoplasts and Nicotiana benthamiana leaf discs have shown that the viral AL2 gene product transactivates expression of the coat protein (CP) and BR1 movement protein genes, and that activation occurs at the level of transcription. Because of its function and properties, we propose the name TrAP, transcriptional activator protein, for the AL2 gene product. Using transgenes consisting of complete and truncated versions of the CP promoter fused to the GUS reporter gene, we show in the studies presented here that TrAP is required for CP gene expression in both mesophyll and phloem tissues. Surprisingly, TrAP appears to induce CP expression by different mechanisms in different cell types: it may activate the CP promoter in mesophyll cells, and acts to derepress the promoter in phloem tissue. In addition, TrAP is clearl...Continue Reading

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Citations

Feb 23, 2007·Molecular Plant-microbe Interactions : MPMI·Surendranath BalijiGarry Sunter
Jun 9, 2007·Molecular Plant-microbe Interactions : MPMI·Kenny BonfimFrancisco J L Aragão
Apr 3, 2003·The Plant Cell·Linhui HaoDavid M Bisaro
Nov 15, 2003·The Plant Cell·Hui WangDavid M Bisaro
Feb 14, 2003·Journal of Virology·Werner Preiss, Holger Jeske
Jun 16, 2005·Journal of Virology·P V ShivaprasadMikhail M Pooggin
Aug 5, 2005·Annual Review of Phytopathology·Maria R RojasRobert L Gilbertson
Feb 26, 2005·Virology Journal·Jeff VeltenChristopher I Cazzonelli
Mar 10, 2011·Virology Journal·Ilenia Rentería-CanettRafael F Rivera-Bustamante
May 5, 2000·Molecular Plant-microbe Interactions : MPMI·I DryA Rezaian
May 3, 2001·Molecular Plant-microbe Interactions : MPMI·K NikovicsG V Horváth
May 18, 2012·Applied Biochemistry and Biotechnology·Jamilur RahmanSunil Kumar Mukherjee
Apr 26, 2013·Molecular Plant Pathology·Vincent N Fondong
May 8, 2010·Molecular Plant Pathology·Carolina EscobarCarmen Fenoll
Sep 20, 2005·Virus Research·Cuiping Guan, Xueping Zhou

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