PMID: 10766917Apr 15, 2000Paper

Regulation of a hyperpolarization-activated chloride current in murine respiratory ciliated cells

The Journal of Physiology
R TarranM A Gray


1. The properties of a hyperpolarization-activated Cl- current (Ihyp-act) in murine ciliated respiratory cells have been studied using whole cell patch clamping. 2. The current-voltage relationship was inwardly rectifying which was due to voltage-dependent gating of the channel. 3. Inward current was markedly sensitive to the extracellular Cl- concentration, an effect that was not related to changes in transmembrane Cl- gradient. Decreasing extracellular Cl- concentration to 6 mM caused a 70 % reduction in inward current with the dose-response relationship exhibiting a Hill coefficient of approximately 2.0 and an IC50 of 29 mM. 4. External anion replacement gave a selectivity sequence of Br- >= I- > Cl- > gluconate = aspartate. The more permeant halides significantly increased current density while the less permeant anions decreased current density, indicating that an extracellular anion is important for channel activity. 5. The conductance was unaffected by exposure to anisotonic pipette solutions or to increases in intracellular cAMP; however, current density was reduced dose dependently by increases in intracellular calcium concentration from 0.1 to 0.5 microM. These results indicate that Ihyp-act is unlikely to be involved ...Continue Reading


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