Regulation of acrosome reaction of fowl spermatozoa: evidence for the involvement of protein kinase C and protein phosphatase-type 1 and/or -type 2A

Reproduction : the Official Journal of the Society for the Study of Fertility
K AshizawaY Tsuzuki

Abstract

The signal transduction pathways involved in the regulation of the acrosome reaction and motility of fowl spermatozoa were investigated. The motility and acrosomal integrity of fowl spermatozoa in TES/NaCl buffer, with or without homogenised inner perivitelline layers (IPVL), prepared from laid fowl eggs, was almost negligible at 40 degrees C. In the presence of 2 mmol CaCl(2)/l at 40 degrees C, motility became vigorous and the acrosome reaction was stimulated when IPVL was added. In the absence of Ca(2+), motility was stimulated by the addition of calyculin A and okadaic acid, both specific inhibitors of protein phosphatase-type 1 (PP1) and -type 2A (PP2A), but Okadaic acid, which is a weaker inhibitor of PP1, did not completely restore motility at 40 degrees C. However, the acrosome reaction was significantly and equally stimulated in a dose-dependent manner by both inhibitors in the range of 10-1000 nmol/l, when spermatozoa were incubated with IPVL but without Ca(2+). These inhibitors did not stimulate the acrosome reaction in the absence of IPVL. The vigorous motility of spermatozoa, stimulated by the addition of Ca(2+), was reduced gradually as the concentrations of SC-9, a selective activator of protein kinase C (PKC), we...Continue Reading

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Citations

Mar 20, 2012·Cell and Tissue Research·Janetti SignorelliPatricio Morales
May 8, 2013·British Poultry Science·R P SinghG B Martin
Mar 20, 2013·BMC Cell Biology·Luis Korrodi-GregórioEdgar F da Cruz E Silva
May 6, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·T RotmanH Breitbart
Jan 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Tomohiro SasanamiMakoto Mori
Jan 9, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·H RotfeldH Breitbart
Jul 11, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·M LemoineE Blesbois
Jun 29, 2010·Theriogenology·Agnieszka PartykaEwa Łukaszewicz
Sep 25, 2009·Molecular Reproduction and Development·Madhavi KaliveDavid G Capco
Dec 17, 2014·Animal Reproduction Science·Chuen-Yu ChengSan-Yuan Huang
Oct 10, 2014·Biology of Reproduction·Thi Mong Diep NguyenElisabeth Blesbois
Jun 12, 2009·Biology of Reproduction·Manuela LemoineElisabeth Blesbois
Sep 20, 2019·International Journal of Molecular Sciences·Patricia Peris-FrauAna Josefa Soler
Jan 25, 2017·The Journal of Poultry Science·Mei MatsuzakiTomohiro Sasanami

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