PMID: 6161233Oct 1, 1980Paper

Regulation of adenosine-sensitive adenylate cyclase from rat brain striatum

Journal of Neurochemistry
M B Anand-Srivastava, R A Johnson

Abstract

An adenosine-sensitive adenylate cyclase has been characterized from rat brain striatum. In whole homogenates as well as in particulate fractions, N6-phenylisopropyl adenosine (PIA), 2-chloroadenosine, and adenosine N'-oxide were equipotent in stimulating adenylate cyclase. Although GTP inhibited basal as well as PIA-stimulated activity of whole homogenates, the enzyme showed an absolute dependency on GTP for stimulation by PIA, dopamine, epinephrine, and norepinephrine in a particulate fraction derived from discontinuous sucrose gradient centrifugation. Adenosine exerts two effects on this adenylate cyclase, stimulation at low concentrations and inhibition at high concentrations, suggesting the presence of two adenosine binding sites. The stimulation of adenylate cyclase by PIA was dependent on the concentration of Mg2+. The degree of stimulation by PIA was greater at a low concentration of MG2+, which suggests that stimulation by PIA was accompanied by increasing the apparent affinity for Mg2+. Activation of adenylate cyclase by PIA was blocked by theophylline or 3-isobutyl-1-methylxanthine (IBMX). The pH optimum for basal or (PIA + GTP)-stimulated activities was broad, with a peak between 8.5 and 9.5. In the presence of GTP,...Continue Reading

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Citations

Mar 1, 1984·Naunyn-Schmiedeberg's Archives of Pharmacology·S M Yeung, R D Green
Jan 1, 1985·Neurochemistry International·M Messripour, J B Clark
Feb 1, 1996·Progress in Neurobiology·A M Sebastião, J A Ribeiro
Jun 1, 1988·Molecular and Cellular Endocrinology·M B Anand-Srivastava
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J L GarciaA M Municio
Jun 1, 1983·Archives of Biochemistry and Biophysics·M B Anand-Srivastava, M Cantin
Oct 1, 1982·Archives of Biochemistry and Biophysics·R A Johnson
Dec 1, 1985·Archives of Biochemistry and Biophysics·M B Anand-Srivastava
Sep 1, 1983·Journal of Neurochemistry·W J Wojcik, N H Neff
May 1, 1987·British Journal of Pharmacology·M Al-Gadi, S J Hill
Feb 1, 1982·Journal of Neurochemistry·R Paes de Carvalho, F G de Mello
May 1, 1981·Journal of Neurochemistry·M B Anand-Srivastava, R A Johnson
Sep 16, 1982·Biochemical and Biophysical Research Communications·M B Anand-SrivastavaJ Genest
Aug 30, 1985·Biochemical and Biophysical Research Communications·J I ChocaR D Green

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