Dec 1, 1991

Regulation of adenylyl cyclase by noradrenaline and tumour necrosis factor alpha in rat cardiomyocytes

European Heart Journal
C ReithmannK Werdan

Abstract

The regulation of adenylyl cyclase components and of adenylyl cyclase activity by noradrenaline and tumour necrosis factor alpha (TNF alpha) was studied in rat cardiomyocytes. Long-term treatment of rat cardiomyocytes in the presence of noradrenaline leads, in addition to a down-regulation of beta 1-adrenoceptors, to an increase in the level of inhibitory G protein alpha-subunits and to a heterologous desensitization of adenylyl cyclase stimulation. Similar to the noradrenaline exposure, incubation of the cardiomyocytes in the presence of the cytokine TNF alpha (10 U.ml-1) also increases the level of Gi alpha proteins. However, in contrast to the noradrenaline treatment, which apparently induces a selective up-regulation of Gi alpha, the TNF alpha exposure also increases the level or activity of other components of adenylyl cyclase, such as the level of membrane beta 36-subunits of G proteins and, most likely, the level of the alpha-subunits of the stimulatory G protein (Gs alpha) and the activity of adenylyl cyclase catalytic subunit. While noradrenaline treatment desensitizes receptor-dependent and independent adenylyl cyclase activity, treatment of the cells with TNF alpha induces a sensitization of adenylyl cyclase stimulat...Continue Reading

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Mentioned in this Paper

Guanosine
ATP8A2 gene
Tumor Necrosis Factor-alpha
GNAS gene
IL17F gene
Membrane
Regulation of Biological Process
Tnf
Receptor Down-Regulation
Neoplasms

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