PMID: 9658197Jul 11, 1998Paper

Regulation of adenylyl cyclase type V/VI in smooth muscle: interplay of inhibitory G protein and Ca2+ influx

Molecular Pharmacology
K S Murthy, G M Makhlouf

Abstract

The characteristics of inhibitory regulation of adenylyl cyclase V/VI by Ca2+ and G proteins were examined in dispersed gastric smooth muscle cells. The mechanisms were evoked separately, sequentially, or concurrently using ligand-gated and G protein-coupled receptor agonists and receptor-independent probes (e. g, thapsigargin). During the initial phase of agonist stimulation, alpha,beta-methylene-ATP, UTP, and ATP inhibited forskolin-stimulated cAMP formation in a concentration-dependent fashion. Inhibition by alpha,beta-methylene-ATP, which activates ligand-gated P2X receptors, was abolished by zero Ca2+, whereas inhibition by UTP, which activates P2Y2 receptors coupled to Gq/11 and Gi3, was not affected by zero Ca2+ but was abolished by pertussis toxin (PTX). Inhibition by ATP, which activates both P2X and P2Y2 receptors, was not affected by zero Ca2+ alone; but after inhibition mediated by Galphai3 was blocked with PTX, inhibition by Ca2+ influx was unmasked and was abolished by zero Ca2+. Inhibition by cholecystokinin-8 was observed only during the phase of capacitative Ca2+ influx and was blocked by zero Ca2+. Inhibition by UTP during this phase was not affected by zero Ca2+ alone; but after inhibition mediated by Galphai...Continue Reading

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Citations

May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·K B JourdanN W Morrell
Jan 30, 2008·Pflügers Archiv : European journal of physiology·Ron W WellsMichael G Blennerhassett
May 2, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gopireddy Raghavender ReddyMoritz Bünemann
Apr 27, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anastasia SobolewskiNicholas W Morrell
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Andrew T ChaytorTudor M Griffith
Apr 10, 2010·Pflügers Archiv : European journal of physiology·Cor de Wit, Tudor M Griffith
Nov 6, 2009·American Journal of Physiology. Cell Physiology·Kathrin von HaynMoritz Bünemann
Dec 19, 2018·Digestive Diseases and Sciences·Bryan A BlakeneyJohn R Grider
Nov 5, 2004·American Journal of Physiology. Cell Physiology·Karnam S MurthySrinivas N Pentyala
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tudor M GriffithDavid H Edwards
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Jul 29, 2004·Molecular and Cellular Endocrinology·T Wang, M J Brown
Mar 15, 2005·Cellular Signalling·Tasmina A Goraya, Dermot M F Cooper

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