Regulation of AE2 mRNA expression in the cortical collecting duct by acid/base balance

The American Journal of Physiology
G Fejes-TóthA Náray-Fejes-Tóth

Abstract

AE2 mRNA and protein is expressed in several nephron segments, one of which is the cortical collecting duct (CCD). However, the distribution of AE2 among the different cell types of the CCD and the function of AE2 in the kidney are not known. The purpose of this study was to determine the distribution of AE2 mRNA among the three CCD cell types and to examine the effects of changes in acid/base balance on its expression. Following NH4Cl (acid) or NaHCO3 (base) loading of rabbits for approximately 18 h, CCD cells were isolated by immunodissection. AE2 mRNA levels were determined by RT-PCR and were normalized for beta-actin levels. We found that CCD cells express high levels of AE2 mRNA (approximately 500 copies/cell). AE2 mRNA levels were significantly higher in CCD cells originating from base-loaded than acid-loaded rabbits, with an average increase of 3.7 +/- 1.07-fold. The effect of pH on AE2 mRNA levels was also tested directly using primary cultures of CCD cells. CCD cells incubated in acidic media expressed significantly lower levels of AE2 mRNA than those in normal or alkaline media. Experiments with isolated principal cells, alpha-intercalated cells, and beta-intercalated cells (separated by fluorescence-activated cell so...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Fejes-Tóth, A Náray-Fejes-Tóth
Jan 1, 1991·Annual Review of Physiology·S L Alper
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A E LindseyR R Kopito
Nov 4, 1985·Nature·G J SchwartzQ Al-Awqati
Sep 1, 1986·The American Journal of Physiology·V L SchusterM L Jennings
Jan 1, 1993·Annual Review of Physiology·V L Schuster
Jan 1, 1997·Pflügers Archiv : European journal of physiology·X HeB J Baum

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Citations

Sep 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jennifer L TuriAndrew J Ghio
Jul 12, 2002·American Journal of Physiology. Renal Physiology·James A Schafer

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