PMID: 2114409Jul 1, 1990Paper

Regulation of anaphase chromosome motion in Tradescantia stamen hair cells by calcium and related signaling agents

The Journal of Cell Biology
D H ZhangP K Hepler

Abstract

Several lines of evidence support the idea that increases in the intracellular free calcium concentration [( Ca2+]i) regulate chromosome motion. To directly test this we have iontophoretically injected Ca2+ or related signaling agents into Tradescantia stamen hair cells during anaphase and measured their effect on chromosome motion and on the Ca2+ levels. Ca2+ at (+)1 nA for 10 s (approximately 1 microM) causes a transient (20 s) twofold increase in the rate of chromosome motion, while at higher levels it slows or completely stops motion. Ca2+ buffers, EGTA, and 5,5'-dibromo-1,2- bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, which transiently suppress the ion level, also momentarily stop motion. Injection of K+, Cl-, or Mg2+, as controls, have no effect on motion. The injection of GTP gamma S, and to a lesser extent GTP, enhances motion similarly to a low level of Ca2+. However, inositol 1,4,5-trisphosphate, ATP gamma S, ATP, and GDP beta S have no effect. Measurement of the [Ca2+]i with indo-1 reveals that the direct injections of Ca2+ produce the expected increases. GTP gamma S, on the other hand, causes only a small [Ca2+]i rise, which by itself is insufficient to increase the rate of chromosome motion. Further studi...Continue Reading

References

Jul 1, 1975·Biochemistry·J B Olmsted, G G Borisy
Jun 30, 1975·Annals of the New York Academy of Sciences·E D Salmon
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E SpeksnijderL F Jaffe
Sep 21, 1989·Nature·M J Berridge, R F Irvine
Dec 1, 1989·The Journal of Cell Biology·P K Hepler
Oct 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H L Yin
Jan 1, 1986·The Journal of Membrane Biology·M C Sekar, L E Hokin
Dec 19, 1986·Science·P W MajerusD B Wilson
Jan 1, 1985·Annual Review of Cell Biology·T P StosselH L Yin
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Sep 1, 1988·The Journal of Cell Biology·R R RatanM L Shelanski
Jun 1, 1986·The Journal of Cell Biology·L U CassimerisE D Salmon
Jan 1, 1987·Cell Motility and the Cytoskeleton·C H Keith
Nov 1, 1987·The Journal of Cell Biology·P K Hepler, D A Callaham
Jun 1, 1986·The Journal of Cell Biology·P K Hepler, B A Palevitz
Aug 1, 1980·The Journal of Cell Biology·E D Salmon, R R Segall

❮ Previous
Next ❯

Citations

Apr 1, 1996·Plant Molecular Biology·W WangB W Poovaiah
Dec 1, 1991·Developmental Biology·K S RathoreK R Robinson
Feb 1, 1991·The Journal of Cell Biology·N Grandin, M Charbonneau
Feb 14, 2013·Theoretical Biology & Medical Modelling·L John Gagliardi, Daniel H Shain
Oct 1, 1994·Cell Calcium·P K Hepler
Nov 4, 2016·Cell Division·L John Gagliardi, Daniel H Shain
Dec 22, 1999·The Plant Journal : for Cell and Molecular Biology·M Tlalka, M Fricker
Jul 10, 1998·FEBS Letters·L DownieD E Evans
Aug 17, 2006·Physics in Medicine and Biology·Steven Babic, L John Schreiner
Jun 14, 2005·Molecular Reproduction and Development·Shu-Zhen LiuDa-Yuan Chen
Mar 19, 2013·The Plant Journal : for Cell and Molecular Biology·Peter K HeplerBrian E S Gunning
Oct 1, 1993·FEMS Microbiology Letters·A T Salek
Dec 12, 1991·Biochimica Et Biophysica Acta·R L Pisoni, J G Thoene

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.