Regulation of angiotensin II receptor subtypes by dexamethasone in rat mesangial cells

Hypertension
D ChanselR Ardaillou

Abstract

The objective of this study was to examine the role of dexamethasone on the expression of angiotensin II (Ang II) receptors in cultured rat mesangial cells. Dexamethasone caused concentration- and time-dependent decreases in 125I-[Sar1,Ala8]Ang II binding that were prevented by glucocorticoid receptor inhibition with mifepristone. A lag time of 24 hours and a dexamethasone concentration of at least 10 nmol/L were necessary for this effect to occur. Dexamethasone-induced reduction of 125I-[Sar1,Ala8]Ang II binding resulted from decreased Ang II type 1 (AT1) receptor density. No change in the apparent dissociation constant was observed. Dexamethasone also markedly inhibited Ang II-dependent inositol phosphate accumulation. Both reverse transcription-polymerase chain reaction and Northern blot analysis using specific short probes from the 3' noncoding region of the cDNA demonstrated the presence of AT1A and AT1B receptor mRNAs in rat mesangial cells, with a slight predominance of AT1B. Therefore, we studied the effect of dexamethasone on the expression of these two subtypes in rat mesangial cells. Dexamethasone produced a time-dependent decrease of AT1B receptor mRNA that was apparent after 6 hours of incubation, whereas AT1A rece...Continue Reading

References

Feb 14, 1992·Biochemical and Biophysical Research Communications·N Iwai, T Inagami
Mar 31, 1992·Biochemical and Biophysical Research Communications·S S KakarJ D Neill
May 29, 1992·Biochemical and Biophysical Research Communications·M Q Ye, D P Healy
Jun 15, 1992·Biochemical and Biophysical Research Communications·S S KakarJ D Neill
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S W LeeA C Allison
Mar 1, 1987·The American Journal of Physiology·J G Douglas
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jan 1, 1995·The Journal of Clinical Investigation·Y NioM Inada
Oct 14, 1994·Biochemical and Biophysical Research Communications·S UnoT Inagami
May 17, 1994·Biochimica Et Biophysica Acta·D F Guo, T Inagami
Sep 1, 1993·Kidney International·G GrandalianoH E Abboud
Dec 15, 1993·Biochemical and Biophysical Research Communications·A T ChiuP Benfield

❮ Previous
Next ❯

Citations

Dec 28, 1999·European Journal of Pharmacology·D ChanselR Ardaillou
Feb 18, 1999·Molecular and Cellular Endocrinology·Y KitamuraT Saruta
Sep 1, 1999·Kidney International·A Hus-CitharelC Llorens-Cortes
May 5, 2009·Journal of Molecular Endocrinology·Irina G BogdarinaAdrian J L Clark
Aug 10, 2011·European Journal of Endocrinology·Maria Teresa RocchettiSalvatore Di Paolo
Jan 14, 1999·Journal of Hypertension·J ZhuoF A Mendelsohn
Oct 1, 1996·Kidney International·S CzekalskiR Ardaillou
Aug 13, 2003·Kidney International·Bettina LanzMarkus G Mohaupt
Aug 15, 2015·Molecular and Cellular Biochemistry·Barbara LewkoJan Stępiński
Mar 14, 2007·Regulatory Peptides·Patrícia Maria FerreiraMaria José Campagnole-Santos
May 12, 2007·Regulatory Peptides·Vardan T Karamyan, Robert C Speth
Aug 1, 2009·Molecular and Cellular Endocrinology·Heidi DemaegdtGeorges Vauquelin
Oct 19, 2001·Endocrinology·A Hus-CitharelC Llorens-Cortes
Jun 1, 2007·American Journal of Physiology. Renal Physiology·Reetu R SinghLuise A Cullen-McEwen
Jul 3, 2003·American Journal of Physiology. Renal Physiology·So Yeon ParkHyun Soon Lee
May 18, 1999·The American Journal of Physiology·F Amiri, R Garcia
Nov 30, 2000·American Journal of Physiology. Renal Physiology·J SchwöbelM Mohaupt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.