Regulation of angiotensin II type 2 receptor gene by the protein kinase C-calcium pathway

Hypertension
K KijimaM Inada

Abstract

In the present study, rat angiotensin II type 2 (AT2) receptor expression was upregulated in confluence-arrested PC12 cells compared with expression in proliferating cells. Treatment with cycloheximide inhibited the increase in mRNA levels in confluent cells. The state of growth arrest by serum deprivation was associated with increased expression of the AT2 receptor, which was markedly suppressed by exposure to the active phorbol ester 12-O-tetradecanoylphorbol 13-acetate and the calcium ionophore A23187. Similar inhibitions were also observed in myocytes isolated from neonatal rat heart. The change in AT2 mRNA levels by serum deprivation was due to the increase in the gene transcription rate. The effect of 12-O-tetradecanoylphorbol 13-acetate was mediated through decreases in gene transcription and mRNA stability, whereas A23187 affected mRNA stability. Vasoactive substances with the protein kinase C-calcium pathway, such as norepinephrine and angiotensin II, also downregulated the AT2 mRNA level in myocytes. These findings indicate that the expression of AT2 receptor in PC12 cells is regulated in a growth state-dependent manner, which is involved in confluence-induced new protein synthesis, thus providing a means by which cel...Continue Reading

References

Jan 1, 1978·Neuroendocrinology·M I Phillips
Feb 28, 1992·Biochemical and Biophysical Research Communications·S P BottariM de Gasparo
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C SumnersM K Raizada
Jan 1, 1991·Endocrinology·K Tsutsumi, J M Saavedra
Nov 30, 1989·Biochemical and Biophysical Research Communications·A T ChiuA L Johnson
Mar 11, 1981·The American Journal of Physiology·M H Saier
Jan 1, 1995·The Journal of Clinical Investigation·Y NioM Inada
Feb 15, 1995·Biochemical and Biophysical Research Communications·M TanakaH Miyazaki
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·H HondermarckR A Bradshaw
Sep 1, 1993·The American Journal of Physiology·J KangP Posner
Nov 1, 1993·The Journal of Clinical Investigation·B E CoxC R Rosenfeld
Mar 19, 1993·Regulatory Peptides·D T Dudley, R M Summerfelt

❮ Previous
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Citations

Feb 10, 2006·European Journal of Applied Physiology·Bruce E BlanchardLinda S Pescatello
Sep 12, 2000·Current Hypertension Reports·R M CareyH M Siragy
Jun 1, 2001·Regulatory Peptides·M Stoll, T Unger
Jul 8, 1999·Regulatory Peptides·M de Gasparo, H M Siragy
Dec 1, 2001·Trends in Cardiovascular Medicine·T Inagami, T Senbonmatsu
Sep 13, 2003·Kidney International. Supplement·Marta Ruiz-OrtegaJesús Egido
Jul 28, 2006·Biochemical and Biophysical Research Communications·F M RollwagenR Maheshwari
Nov 25, 1998·Biochemical and Biophysical Research Communications·P ZahradkaL Saward
Nov 5, 1997·Biochemical and Biophysical Research Communications·K ShibataT Furukawa
Jan 31, 2015·Journal of Cardiovascular Pharmacology·Luis C Matavelli, Helmy M Siragy
Jul 19, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Christophe AdamyCatherine Chassagne
Aug 5, 2000·Journal of Applied Physiology·H KraicziJ Carlson
Jul 23, 2003·Journal of Hypertension·Massimo VolpeAlberto Morganti

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