Regulation of ANGPTL8 in liver and adipose tissue by nutritional and hormonal signals and its effect on glucose homeostasis in mice

American Journal of Physiology. Endocrinology and Metabolism
Lu ZhangLuke Norton

Abstract

The angiopoietin-like protein (ANGPTL) family represents a promising therapeutic target for dyslipidemia, which is a feature of obesity and type 2 diabetes (T2DM). The aim of the present study was to determine the metabolic role of ANGPTL8 and to investigate its nutritional, hormonal, and molecular regulation in key metabolic tissues. The regulation of Angptl8 gene expression by insulin and glucose was quantified using a combination of in vivo insulin clamp experiments in mice and in vitro experiments in primary and cultured hepatocytes and adipocytes. The role of AMPK signaling was examined, and the transcriptional control of Angptl8 was determined using bioinformatic and luciferase reporter approaches. The metabolism of Angptl8 knockout mice (ANGPTL8-/-) was examined following chow and high-fat diets (HFD). Insulin acutely increased Angptl8 expression in liver and adipose tissue, which involved the CCAAT/enhancer-binding protein (C/EBPβ) transcription factor. In insulin clamp experiments, glucose further enhanced Angptl8 expression in the presence of insulin in adipose tissue. The activation of AMPK signaling antagonized the effect of insulin on Angptl8 expression in hepatocytes and adipocytes. The ANGPTL8-/- mice had improve...Continue Reading

References

Jun 1, 1988·Metabolism: Clinical and Experimental·M KuwajimaJ D McGarry
Aug 2, 2003·The Journal of Biological Chemistry·Suzanne KovacicJason R B Dyck
Jun 17, 2004·Circulation·Stefano BellostaAlberto Corsini
Aug 25, 2009·Biochemical and Biophysical Research Communications·Veronica LopezJheem D Medh
Mar 6, 2010·Methods in Molecular Biology·Wan-Chun LiDavid Tosh
Oct 15, 2010·The New England Journal of Medicine·Kiran MusunuruSekar Kathiresan
Mar 20, 2012·Analytical Biochemistry·Katja ZebischMatthias Brandsch
May 10, 2012·American Journal of Physiology. Endocrinology and Metabolism·Gang RenCynthia M Smas
Mar 19, 2013·Journal of Medical Genetics·Daphna Weissglas-VolkovPäivi Pajukanta
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yan WangHelen H Hobbs
May 24, 2014·Scientific Reports·Zhiyao FuRen Zhang
Jul 23, 2014·The Journal of Clinical Endocrinology and Metabolism·Javier Gómez-AmbrosiGema Frühbeck
Nov 22, 2014·Nucleic Acids Research·Luke NortonSami Heikkinen
Nov 25, 2014·Cell·Viktoria GusarovaJesper Gromada
Mar 11, 2015·Endocrine Journal·Hodaka YamadaSan-E Ishikawa
Jul 24, 2015·The Journal of Clinical Endocrinology and Metabolism·P A Nidhina HaridasHannele Yki-Järvinen
Apr 2, 2016·Journal of Lipid Research·Wieneke DijkSander Kersten
Jul 30, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Bruno Martinez-PerezAna Megía
Jul 19, 2017·Scientific Reports·Puhan LuXuefeng Yu
Jul 20, 2017·Diabetes/metabolism Research and Reviews·Mohamed Abu-FarhaJaakko Tuomilehto
Oct 17, 2017·Molecular Metabolism·Xun ChiBrandon S J Davies
Jan 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Serena BanfiHelen H Hobbs
Apr 6, 2018·Diabetologia·Brandon S J Davies
Jul 20, 2018·The Journal of Biological Chemistry·Wieneke DijkSander Kersten
Jan 29, 2019·Journal of Lipid Research·Oleg KovrovGunilla Olivecrona
Aug 25, 2019·Molecular Metabolism·N JaiswalP M Titchenell

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Citations

Feb 24, 2021·The Journal of Clinical Endocrinology and Metabolism·Federico OldoniHelen H Hobbs
May 1, 2021·International Journal of Molecular Sciences·Hyunmi KimKyoung-Jin Oh
May 24, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xin SuBin Wang
Sep 29, 2021·Open Biology·Chang GuoGuoyue Yuan

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Methods Mentioned

BETA
antisense oligonucleotides
PCR
Transfection
ELISA

Software Mentioned

PrimerQuest Design Tool
xPONENT

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