Regulation of angR, a gene with regulatory and biosynthetic functions in the pJM1 plasmid-mediated iron uptake system of Vibrio anguillarum

Gene
P Salinas, J H Crosa

Abstract

AngR and the product(s) encoded in the trans-acting factor (TAF) region are necessary for the full expression of the pJM1 plasmid-mediated anguibactin iron-uptake system in Vibrio anguillarum (Va). In this report, we analyzed the factors that affect the expression of the angR gene. Northern blot analysis showed that angR encodes a 3.1-kb transcript which is expressed only under iron-limiting conditions. Measurement of steady-state RNA levels show that, under iron-limiting conditions, angR is positively regulated at the transcriptional level by product(s) of the Va TAF region. However, this enhancement of angR expression by TAF does not occur at high levels of the AngR protein, as assessed by using an angR::lacZ fusion in the presence of a construct containing angR under the control of ptac. We also report that repression of angR by iron could possibly be mediated by an endogenous Va antisense RNA beta, which contains a stem-loop structure complementary to the stem-loop structure located at the 5' end of angR.

References

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Citations

Dec 4, 2002·Plasmid·Michiel StorkJorge H Crosa
Sep 18, 2003·Journal of Bacteriology·Manuela Di LorenzoJorge H Crosa
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Jorge H Crosa, Christopher T Walsh
Apr 12, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Daniel McIntosh-TolleJorge H Crosa
May 11, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Hiroaki NakaJorge H Crosa
Dec 15, 2015·Microbiology and Molecular Biology Reviews : MMBR·Shelley M PayneElizabeth E Wyckoff
Aug 10, 2017·Frontiers in Cellular and Infection Microbiology·Yingjie Li, Qingjun Ma
Nov 24, 1999·Infection and Immunity·A M WertheimerJ H Crosa
Jun 25, 2015·Microbiology Spectrum·Manuela Di Lorenzo, Michiel Stork
Sep 18, 1997·Microbiology and Molecular Biology Reviews : MMBR·J H Crosa

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