Regulation of anion secretion by prostaglandin E2 in the mouse endometrial epithelium

Biology of Reproduction
S K Fong, Hsiao Chang Chan

Abstract

The present study was an investigation of the regulation of anion secretion across cultured mouse endometrial epithelium by prostaglandin E2 (PGE2) using the short-circuit current (ISC) technique. The cultured endometrial monolayers responded to both apical and basolateral application of PGE2 with a sustained rise in ISC in a concentration-dependent manner. However, the potencies of apical and basolateral addition of PGE2 were different, with apparent EC50 of 200 and 4 nM, respectively. Replacement of Cl- or HCO3- in the bathing solution significantly reduced the ISC responses to both apical and basolateral addition of PGE2; however, the apical response exhibited greater dependence on HCO3- . Pretreatment with diphenylamine 2,2'-dicarboxylic acid, a Cl- channel blocker, significantly reduced both PGE2-induced ISC responses, while pretreatment with amiloride, a Na+ channel blocker, did not exert any effect. Forskolin, an adenylate cyclase activator, and 3-isobutyl-dihydro-testosterone-1-methyl-xanthine, a cAMP phosphodiesterase inhibitor, mimicked the ISC response to PGE2 while MDL12330A, an adenylate cyclase inhibitor, completely abolished the PGE2-induced ISC. The results of the present study indicate that the anion secretion ...Continue Reading

Citations

Mar 5, 2014·Human Reproduction Update·Ye Chun RuanHsiao Chang Chan
May 19, 2010·The Journal of Physiology·Ruth W Muchekehu, Paul M Quinton
Jan 18, 2006·Molecular and Cellular Endocrinology·Hsiao Chang ChanYu Ying Yuan
Mar 28, 2017·Clinical and Experimental Pharmacology & Physiology·Hui Chen, Hsiao Chang Chan
Aug 24, 1999·Biology of Reproduction·S J DowningH J Leese
Oct 9, 2004·Human Reproduction·Xiao-Ying ZhengHai-Yan Wang
Apr 17, 2021·PLoS Neglected Tropical Diseases·Jian-Bang XuYi-Lin Zhang

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