Regulation of antiprotease and antimicrobial protein secretion by airway submucosal gland serous cells.

The Journal of Biological Chemistry
Nam Soo JooJ J Wine

Abstract

Airway submucosal gland serous cells express the cystic fibrosis transmembrane conductance regulator (CFTR) and secrete antimicrobial, anti-inflammatory, and antioxidant molecules. In cystic fibrosis, diminished gland secretion may impair innate airway host defenses. We used Calu-3 cells as a serous cell model to study the types of proteins released, the pathways that release them, and the possible involvement of CFTR activity in protein release. Many proteins were secreted constitutively into the apical fluid and showed increased release to agonists. We identified some of them by high pressure liquid chromatography-mass spectrometry and reverse transcriptase PCR, including lysozyme, siderocalin (the protein NGAL), which inhibits bacterial growth by binding iron-containing siderophores, HSC-71, which is thought to have anti-inflammatory properties, and the serine protease inhibitors alpha-1-antitrypsin and alpha-1-antichymotrypsin, which may function as antimicrobials as well as play a potential role in diminishing the activation of epithelial Na(+) channels by serine proteases. We used an enzyme-linked immunosorbent assay to quantify lysozyme secretion by Calu-3 cells in response to various agonists and inhibitors. Forskolin i...Continue Reading

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Citations

Mar 28, 2007·Thorax·Christopher H Goss, Jane L Burns
May 4, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hyung-Ju ChoJeffrey J Wine
Mar 1, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·J H WiddicombeSara Modlin
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Sep 30, 2006·American Journal of Respiratory Cell and Molecular Biology·Xiaoming LiuJohn F Engelhardt
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Mar 4, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Derek B McMahonRobert J Lee

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