Regulation of arachidonic acid, eicosanoid, and phospholipase A2 levels in murine mast cells by recombinant stem cell factor

The Journal of Clinical Investigation
A N FontehF H Chilton

Abstract

The current study evaluates the capacity of recombinant rat stem cell factor (rrSCF) to regulate enzymes that control AA release and eicosanoid generation in mouse bone marrow-derived mast cells (BMMCs). Initial studies indicated that rrSCF provided for 24 h inhibited the release of AA into supernatant fluids of antigen- and ionophore A23187-stimulated BMMCs. Agonist-induced increases in cellular levels of AA were also inhibited, albeit to a lesser degree by rrSCF. To determine the inhibitory mechanism, several steps (e.g., mobilization of cytosolic calcium, release of BMMC granules, and regulation of phospholipase A2 [PLA2] activity) that could influence AA release were measured in rrSCF-treated cells. rrSCF did not alter the capacity of BMMCs to mobilize cytosolic calcium or release histamine in response to antigen and ionophore. BMMCs released large amounts of PLA2 with characteristics of the group II family in response to antigen and ionophore A23187. rrSCF treatment of BMMCs reduced the secretion of this PLA2 activity by BMMCs. Partial purification of acid-extractable PLA2 from rrSCF-treated and untreated BMMCs suggested that rrSCF decreased the quantity of acid-stable PLA2 within the cells. In contrast to group II PLA2, t...Continue Reading

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Citations

Aug 7, 1999·Clinical Reviews in Allergy & Immunology·M C Seeds, D A Bass
Nov 18, 2000·Biochimica Et Biophysica Acta·T J NevalainenJ M Grönroos
Mar 12, 2010·Biochimie·Francescopaolo GranataMassimo Triggiani
Sep 13, 2006·Journal of Neuroinflammation·Timothy J CunninghamLihua Yao
Nov 9, 2005·The Journal of Allergy and Clinical Immunology·Massimo TriggianiGianni Marone
Mar 14, 2003·Rheumatology International·Ihsan ErtenliMeral Calgüneri
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A N FontehF H Chilton
Jan 11, 2018·Nanomedicine : Nanotechnology, Biology, and Medicine·Raghavendra GowdaGavin P Robertson

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