Regulation of ATP production: dependence on calcium concentration and respiratory state

American Journal of Physiology. Cell Physiology
Brian D FinkWilliam I Sivitz

Abstract

Nanomolar free calcium enhances oxidative phosphorylation. However, the effects over a broad concentration range, at different respiratory states, or on specific energy substrates are less clear. We examined the action of varying [Ca(2+)] over respiratory states ranging 4 to 3 on skeletal muscle mitochondrial respiration, potential, ATP production, and H2O2 production using ADP recycling to clamp external [ADP]. Calcium at 450 nM enhanced respiration in mitochondria energized by the complex I substrates, glutamate/malate (but not succinate), at [ADP] of 4-256 µM, but more substantially at intermediate respiratory states and not at all at state 4. Using varied [Ca(2+)], we found that the stimulatory effects on respiration and ATP production were most prominent at nanomolar concentrations, but inhibitory at 10 µM or higher. ATP production decreased more than respiration at 10 µM calcium. However, potential continued to increase up to 10 µM; suggesting a calcium-induced inability to utilize potential for phosphorylation independent of opening of the mitochondrial permeability transition pore (MTP). This effect of 10 µM calcium was confirmed by direct determination of ATP production over a range of potential created by differing su...Continue Reading

References

Aug 29, 1978·Biochemical and Biophysical Research Communications·E J Davis, W I Davis-van Thienen
Aug 13, 1976·Biochimica Et Biophysica Acta·U KüsterW Kunz
Apr 1, 1990·Physiological Reviews·J G McCormackR M Denton
May 9, 1980·Biochimica Et Biophysica Acta·F BrawandP Walter
Sep 1, 1984·Archives of Biochemistry and Biophysics·E J Davis, W I Davis-Van Thienen
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Feb 9, 2000·American Journal of Physiology. Cell Physiology·P R TerritoR S Balaban
Feb 29, 2000·Biochimica Et Biophysica Acta·N I KavanaghM D Brand
Aug 12, 2004·Free Radical Biology & Medicine·Martin D BrandNadeene Parker
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Paul S BrookesShey-Shing Sheu
Oct 25, 2006·The Journal of Biological Chemistry·Yunxia O'MalleyWilliam I Sivitz
Jun 8, 2010·Molecules and Cells·Sue Goo RheeDongmin Kang
Jul 6, 2011·The Biochemical Journal·Martin D Brand, David G Nicholls
Nov 30, 2011·Molecular and Cellular Endocrinology·György CsordásGyörgy Hajnóczky
Mar 2, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Darci PhillipsRobert S Balaban
Mar 27, 2012·Biochemistry·Brian Glancy, Robert S Balaban
Sep 1, 1994·Journal of Biomolecular NMR·B A Johnson, R A Blevins
Jan 19, 2013·Diabetes·Liping YuWilliam I Sivitz
Oct 15, 2013·Journal of Bioenergetics and Biomembranes·Liping YuWilliam I Sivitz
Oct 11, 2014·The Journal of Pharmacology and Experimental Therapeutics·Brian D FinkWilliam I Sivitz
Jan 30, 2015·Methods in Molecular Biology·Liping YuWilliam I Sivitz
Mar 31, 2017·Pharmacology Research & Perspectives·Brian D FinkWilliam I Sivitz

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Citations

Jan 8, 2019·PLoS Computational Biology·Sathyavani MalyalaJason N Bazil
Jul 10, 2020·Cell Biochemistry and Function·Feng WangXiang-Hong Ou
Sep 15, 2019·Nature Communications·Sofiya GanchevaMichael Roden
May 13, 2020·Journal of Clinical Medicine·Rosita A CondorelliSandro La Vignera
Oct 22, 2020·International Journal of Molecular Sciences·Lo Lai, Hongyu Qiu
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Feng WangXiang-Hong Ou
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Camille DejosAnna Rita Cantelmo
Mar 12, 2021·Journal of Cellular Physiology·Feng WangXiang-Hong Ou
Mar 20, 2018·Current Opinion in Physiology·Vineeta TanwarLoren E Wold
Apr 27, 2021·Applied Sciences·Jasiel O Strubbe-RiveraJason N Bazil

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Methods Mentioned

BETA
Assay
NMR

Software Mentioned

GraphPad
NMRPipe
GraphPad Prism
Oxygraph
NMRView

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