PMID: 6982304Nov 1, 1982Paper

Regulation of azophenylarsonate-specific repertoire expression. 1. Frequency of cross-reactive idiotype-positive B cells in A/J and BALB/c mice

The Journal of Experimental Medicine
N H Sigal

Abstract

A large proportion of p-azophenylarsonate (ARS)-specific antibodies from A/J mice share a cross-reactive idiotype (CRIA) that comprises a family of closely related but nonidentical clonotypes. I determined that only 2.6 % (7 out of 267) A/J ARS-specific monoclonal antibodies generated in the splenic focus system possess the predominant CRIA. Because ARS-specific B cells are present at a frequency of 1/68,000 B cells, the frequency of the entire idiotype family is 1 per 2.8 X 10(6) splenic B cells. Thus, there is a striking discrepancy between the representation of this idiotype at the clonal precursor cell level and the serum antibody response. In addition, BALB/c mice have the potential to generate CRIA-positive precursor cells within their nonimmune repertoire. When A/J mice are immunized with ARS-protein conjugates, the serum antibody response and precursor cell population are both dominated by CRIA. The frequency of CRIA-positive B cells increases over 100-fold after immunization, whereas CRIA-negative precursor cells may initially decrease, followed by a later rise in frequency. Finally, although ARS-specific precursor cells are present in high frequency at birth, CRIA-positive monoclonal anti-ARS antibodies are not observ...Continue Reading

References

Jan 1, 1978·The Journal of Experimental Medicine·M P CancroN R Klinman
Oct 1, 1977·The Journal of Experimental Medicine·N H SigalN R Klinman
Aug 1, 1972·The Journal of Experimental Medicine·N R Klinman
Dec 1, 1981·European Journal of Immunology·Y Ben-NeriahD Givol
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A Marshak-RothsteinM L Gefter
May 1, 1980·The Journal of Experimental Medicine·K E SteinW E Paul

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Citations

May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S K DattaR S Schwartz
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R I NearM L Gefter
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K S HathcockR J Hodes
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D TeitelbaumR Arnon
Jul 1, 1983·The Journal of Experimental Medicine·M A ThompsonM P Cancro
Jul 1, 1984·The Journal of Experimental Medicine·M SlaouiJ Urbain
Nov 1, 1985·The Journal of Experimental Medicine·B G Froscher, N R Klinman
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M MoserJ Urbain
Apr 1, 1986·Immunological Reviews·M SlaouiJ Urbain

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