Regulation of BCL-6 gene expression in human myeloid/monocytoid leukemic cells

Leukemia
T YamochiM Moriyama

Abstract

We demonstrated in the present study that the BCL-6 transcripts were detectable not only in B cells, but also in circulating granulocytes and monocytes from normal individuals, and in human acute nonlymphocytic leukemia cells of certain subtypes (M3, M4, M5). Then, with an assumption that the BCL-6 gene expression may be related to the differentiation of myeloid cells, we analyzed the inducibility of BCL-6 gene expression along monocytic lineage differentiation in HL-60 and U-937 cells by treating them with 12-O-tetradecanoylphorbol-13-acetate (TPA). Although the expression of BCL-6 transcripts was very low or undetectable in untreated HL-60 or U-937 cells, treatment of these cells with TPA to induce monocytic differentiation resulted in an apparent increase of BCL-6 mRNA, suggesting that BCL-6 gene expression is not limited to B cells and it is closely associated with monocytic lineage differentiation. The BCL-6 transcripts in TPA-treated U-937 cells were superinduced by the treatment with cycloheximide (CHX) and the half-life of the BCL-6 mRNA was apparently prolonged when TPA-treated U-937 cells were exposed to CHX in the presence of actinomycin D (ACD). Furthermore, the nuclear run-on assay revealed that the BCL-6 transcrip...Continue Reading

Citations

Feb 22, 2002·Biochemical and Biophysical Research Communications·Chizuko SakashitaTohru Miki
Jan 8, 2000·International Reviews of Immunology·L M StaudtX Yu
Sep 11, 2009·Human Pathology·Faisal A KhokharFrancisco Vega
Dec 15, 2004·Experimental and Molecular Pathology·Gang Zhou, Santa Jeremy Ono
Jan 28, 1999·Differentiation; Research in Biological Diversity·O Albagli-CurielC Pinset
Jan 1, 2004·The Journal of Biological Chemistry·Matthias LaudesStephen O'Rahilly
Mar 23, 2001·Nature Immunology·S Canfield, P Rothman
Mar 23, 2001·Nature Immunology·L M ToneyA L Dent
Feb 18, 2004·Oncogene·David K CrockettMegan S Lim
Dec 7, 2005·Oncogene·A ChattopadhyayP Ko Ferrigno
Jan 30, 2003·Oncogene·Olivier Albagli-Curiel
Apr 1, 2003·Molecular and Cellular Biology·Leonard C EdelsteinCéline Gélinas
Apr 30, 2005·Journal of Cell Science·Fiona J PixleyB Hilda Ye

❮ Previous
Next ❯

Related Concepts

Related Feeds

AML: Role of LSD1 by CRISPR (Keystone)

Find the latest rersearrch on the ability of CRISPR-Cas9 mutagenesis to profile the interactions between lysine-specific histone demethylase 1 (LSD1) and chemical inhibitors in the context of acute myeloid leukemia (AML) here.

Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous disease with approximately 20,000 cases per year in the United States. AML also accounts for 15-20% of all childhood acute leukemias, while it is responsible for more than half of the leukemic deaths in these patients. Here is the latest research on this disease.

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.