Abstract
We demonstrated in the present study that the BCL-6 transcripts were detectable not only in B cells, but also in circulating granulocytes and monocytes from normal individuals, and in human acute nonlymphocytic leukemia cells of certain subtypes (M3, M4, M5). Then, with an assumption that the BCL-6 gene expression may be related to the differentiation of myeloid cells, we analyzed the inducibility of BCL-6 gene expression along monocytic lineage differentiation in HL-60 and U-937 cells by treating them with 12-O-tetradecanoylphorbol-13-acetate (TPA). Although the expression of BCL-6 transcripts was very low or undetectable in untreated HL-60 or U-937 cells, treatment of these cells with TPA to induce monocytic differentiation resulted in an apparent increase of BCL-6 mRNA, suggesting that BCL-6 gene expression is not limited to B cells and it is closely associated with monocytic lineage differentiation. The BCL-6 transcripts in TPA-treated U-937 cells were superinduced by the treatment with cycloheximide (CHX) and the half-life of the BCL-6 mRNA was apparently prolonged when TPA-treated U-937 cells were exposed to CHX in the presence of actinomycin D (ACD). Furthermore, the nuclear run-on assay revealed that the BCL-6 transcrip...Continue Reading
Citations
Aug 14, 2002·Cancer·Po-Min ChenJin-Hwang Liu
Feb 13, 2001·Leukemia Research·Y WangE Liliemark
Jun 11, 2005·Cell Death and Differentiation·M MontanariS Ferrari
Feb 22, 2002·Biochemical and Biophysical Research Communications·Chizuko SakashitaTohru Miki
Jan 8, 2000·International Reviews of Immunology·L M StaudtX Yu
Dec 30, 2011·Cytokine·Daniel SalamonEva Klein
Sep 11, 2009·Human Pathology·Faisal A KhokharFrancisco Vega
Dec 15, 2004·Experimental and Molecular Pathology·Gang Zhou, Santa Jeremy Ono
Aug 4, 2005·Molecular Immunology·Maki OhtaniShingo Hiroishi
Jan 28, 1999·Differentiation; Research in Biological Diversity·O Albagli-CurielC Pinset
Feb 13, 2001·Genomics·Y SaikiT Nakamura
May 16, 2014·RNA Biology·Xueqing ZhangPeter E Newburger
Sep 3, 2010·Blood·Yukiya YamamotoNobuhiko Emi
Mar 24, 2009·Immunity·Francis CoffeyTim Manser
Jan 1, 2004·The Journal of Biological Chemistry·Matthias LaudesStephen O'Rahilly
Sep 22, 1999·Oncogene·O AlbagliC Lindon
Feb 2, 1999·Oncogene·T YamochiM Moriyama
Mar 23, 2001·Nature Immunology·S Canfield, P Rothman
Mar 23, 2001·Nature Immunology·L M ToneyA L Dent
Jul 26, 2003·Oncogene·Tetsuya KurosuOsamu Miura
Feb 18, 2004·Oncogene·David K CrockettMegan S Lim
Dec 7, 2005·Oncogene·A ChattopadhyayP Ko Ferrigno
Jan 30, 2003·Oncogene·Olivier Albagli-Curiel
Apr 1, 2003·Molecular and Cellular Biology·Leonard C EdelsteinCéline Gélinas
Apr 30, 2005·Journal of Cell Science·Fiona J PixleyB Hilda Ye