Regulation of BDNF chromatin status and promoter accessibility in a neural correlate of associative learning

Epigenetics : Official Journal of the DNA Methylation Society
Ganesh AmbigapathyJoyce Keifer

Abstract

Brain-derived neurotrophic factor (BDNF) gene expression critically controls learning and its aberrant regulation is implicated in Alzheimer's disease and a host of neurodevelopmental disorders. The BDNF gene is target of known DNA regulatory mechanisms but details of its activity-dependent regulation are not fully characterized. We performed a comprehensive analysis of the epigenetic regulation of the turtle BDNF gene (tBDNF) during a neural correlate of associative learning using an in vitro model of eye blink classical conditioning. Shortly after conditioning onset, the results from ChIP-qPCR show conditioning-dependent increases in methyl-CpG-binding protein 2 (MeCP2) and repressor basic helix-loop-helix binding protein 2 (BHLHB2) binding to tBDNF promoter II that corresponds with transcriptional repression. In contrast, enhanced binding of ten-eleven translocation protein 1 (Tet1), extracellular signal-regulated kinase 1/2 (ERK1/2), and cAMP response element-binding protein (CREB) to promoter III corresponds with transcriptional activation. These actions are accompanied by rapid modifications in histone methylation and phosphorylation status of RNA polymerase II (RNAP II). Significantly, these remarkably coordinated change...Continue Reading

References

Sep 21, 2001·Molecular and Cellular Biology·A S WeinmannP J Farnham
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 21, 2004·Brain, Behavior and Evolution·Dantong Zhu, Joyce Keifer
Apr 6, 2006·Cellular and Molecular Life Sciences : CMLS·J M Levenson, J D Sweatt
May 19, 2006·Nature Reviews. Genetics·Thierry Bienvenu, Jamel Chelly
Feb 1, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xueying JiangRobert H Lipsky
Aug 19, 2008·Neurobiology of Learning and Memory·Pirjo KomulainenRainer Rauramaa
Oct 17, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Farah D LubinJ David Sweatt
Nov 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joyce KeiferTimothy G Clark
Jul 23, 2011·Frontiers in Molecular Neuroscience·Joyce Keifer, James C Houk
May 24, 2013·Nature Reviews. Molecular Cell Biology·William A PastorAnjana Rao
Jun 22, 2013·Genes & Development·Philipp VoigtDanny Reinberg
Sep 27, 2013·Genes & Cancer·Pierre-François CartronFrançois M Vallette
Jan 21, 2014·Journal of Molecular Neuroscience : MN·Ganesh AmbigapathyJoyce Keifer
Apr 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xiang LiTimothy W Bredy
May 3, 2014·Integrative and Comparative Biology·Jessica A Head

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Citations

Feb 18, 2017·Genes·Joyce Keifer
Jun 28, 2018·Briefings in Bioinformatics·Dongyang LiuYongchun Zuo
Sep 14, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wookbong KwonZae Young Ryoo
May 17, 2019·Neural Plasticity·Chao-Chao YuLi-Hong Kong
Oct 8, 2020·Journal of Molecular Neuroscience : MN·Zhaoqing Zheng, Joyce Keifer
Jun 29, 2021·Nucleic Acids Research·Adel B AlharbiJohn E J Rasko

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Methods Mentioned

BETA
PCR
ChIP
co-immunoprecipitation
co-IPs
immunoprecipitation

Software Mentioned

StatView
Methprimer

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