PMID: 8594418Jul 19, 1995Paper

Regulation of bovine rod outer segment membrane guanylate cyclase by ATP, phosphodiesterase and metal ions

Molecular and Cellular Biochemistry
A SitaramayyaR K Sharma

Abstract

In vertebrate retina, rod outer segment is the site of visual transduction. The inward cationic current in the dark-adapted outer segment is regulated by cyclic GMP. A light flash on the outer segment activates a cyclic GMP phosphodiesterase resulting in rapid hydrolysis of the cyclic nucleotide which in turn causes a decrease in the dark current. Restoration of the dark current requires inactivation of the phosphodiesterase and synthesis of cyclic GMP. The latter is accomplished by the enzyme guanylate cyclase which catalyzes the formation of cyclic GMP from GTP. Therefore, factors regulating the cyclase activity play a critical role in visual transduction. But regulation of the cyclase by some of these factors--phosphodiesterase, ATP, the soluble proteins and metal cofactors (Mg and Mn)--is controversial. The availability of different types of cyclase preparations, dark-adapted rod outer segments with fully inhibited phosphodiesterase activity, partially purified cyclase without PDE contamination, cloned rod outer segment cyclase free of other rod outer segment proteins, permitted us to address these controversial issues. The results show that ATP inhibits the basal cyclase activity but enhances the stimulation of the enzyme ...Continue Reading

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Citations

Nov 2, 2005·Biochemical and Biophysical Research Communications·Matsuyo YamazakiAkio Yamazaki
Apr 10, 1999·The Journal of Biological Chemistry·E V OlshevskayaA M Dizhoor
Aug 28, 1999·The Journal of Biological Chemistry·E V OlshevskayaA M Dizhoor
Jun 12, 2003·The Journal of Biological Chemistry·Akio YamazakiRussell K Yamazaki

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