Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: isolation of regulatory mutants.

Journal of Bacteriology
J M CalvoH E Umbarger

Abstract

5',5',5'-Trifluoro-dl-leucine inhibited the activity of alpha-isopropylmalate synthetase (the initial enzyme unique to leucine biosynthesis) as well as the growth of Salmonella typhimurium. Mutants of S. typhimurium resistant to the analogue were isolated and characterized. In most cases, they overproduced and excreted leucine or leucine, valine, and isoleucine as a result of an alteration in the regulation of branched-chain amino acid biosynthesis. Biochemical and genetic tests allowed the mutants to be grouped into three classes: I, a moderately large group (13%) which had high, constitutive leucine biosynthetic enzyme levels and mutant sites linked to the leucine operon (operator constitutive); II, a single mutant in which the mutant site was linked to the leucine operon and in which alpha-isopropylmalate synthetase was not inhibited by leucine (feedback negative); III, a majority type which had constitutive levels of leucine, valine, and isoleucine biosynthetic enzymes and mutant sites unlinked to the leucine operon. Mutants of class I provide important evidence for the concept of an operon organization of genes involved in leucine biosynthesis. The properties of class III mutants indicate that there is some element involve...Continue Reading

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Citations

Jun 1, 1972·Biochemical Genetics·C J BrooksD E Catcheside
May 1, 1987·Molecular & General Genetics : MGG·E R Rosenthal, J M Calvo
Aug 1, 1979·Molecular & General Genetics : MGG·D R Smith, J M Calvo
Jan 1, 1983·Molecular & General Genetics : MGG·G Bogosian, R Somerville
Jan 1, 1985·Molecular & General Genetics : MGG·D FriedbergJ M Calvo
Feb 11, 1998·Journal of Bacteriology·M Fang, H Y Wu
Sep 1, 1971·Journal of Bacteriology·M KisumiI Chibata
Jul 1, 1972·Journal of Bacteriology·R Twarog
Apr 1, 1971·Journal of Bacteriology·R R AlexanderM Freundlich
Jun 1, 1971·Journal of Bacteriology·T Tosa, L I Pizer
Aug 1, 1972·Journal of Bacteriology·J P O'Neill, M Freundlich
Dec 1, 1972·Bacteriological Reviews·K E Sanderson
Jun 1, 1983·Journal of Bacteriology·C H SquiresJ M Calvo
Feb 1, 1984·Journal of Bacteriology·J C GarbeP S Vary
Oct 1, 1971·Journal of Bacteriology·B Stieglitz, J M Calvo
Jun 1, 1970·Bacteriological Reviews·K E Sanderson
Jul 1, 1985·Journal of Bacteriology·G W HaughnJ M Calvo
Nov 1, 1973·Journal of Bacteriology·J B Ward, S A Zahler
Nov 1, 1973·Journal of Bacteriology·J B Ward, S A Zahler
Feb 1, 1977·Journal of Bacteriology·M G Davis, J M Calvo
Jul 1, 1983·Journal of Bacteriology·K T HughesB M Olivera
Jul 15, 1981·Journal of Molecular Biology·S R Wessler, J M Calvo
Apr 5, 1987·Journal of Molecular Biology·E R Rosenthal, J M Calvo

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