Regulation of Ca2+ handling by phosphorylation status in mouse fast- and slow-twitch skeletal muscle fibers

The American Journal of Physiology
Y LiuM F Schneider

Abstract

The effects of phosphorylation status on Ca2+ release and Ca2+ removal were studied in fast-twitch flexor digitorum brevis and slow-twitch soleus skeletal muscle fibers enzymatically isolated from wild-type and phospholamban knockout (PLBko) mice. In all fibers the adenosine 3',5'-cyclic monophosphate-dependent protein kinase (PKA) inhibitor H-89 decreased the peak amplitude of the intracellular Ca2+ concentration ([Ca2+]) transient for a single action potential, and the PKA activator dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) reversed this effect, indicating modulation of Ca2+ release by phosphorylation status in all fibers. H-89 decreased the decay rate constant of the [Ca2+] transient and DBcAMP reversed this effect only in phospholamban-expressing fibers (wild-type soleus), indicating modulation of Ca2+ removal only in the presence of phospholamban. A high basal level of PKA phosphorylation in soleus fibers maintained under our control conditions was indicated by the lack of effect of direct application of DBcAMP on Ca2+ release or Ca2+ removal in wild-type or PLBko soleus fibers and was confirmed by analysis of phospholamban from wild-type soleus fibers.

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Citations

Jun 29, 2006·Diabetes·Johanna T LannerHåkan Westerblad
Dec 20, 2007·The Journal of Biological Chemistry·Benjamin L ProsserMartin F Schneider
Jan 15, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Johanna T LannerHåkan Westerblad
Apr 16, 2020·The Journal of General Physiology·Francine UweraJean-Marc Renaud
Nov 11, 2003·Canadian Journal of Physiology and Pharmacology·S A ReadingJ K Barclay
Apr 3, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qiujing SongEvangelia G Kranias
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May 19, 2009·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·A Russell Tupling
Sep 25, 2015·The Journal of Physiology·Simeon P Cairns, Fabio Borrani
Feb 10, 2004·The Journal of Biological Chemistry·Patricia RodriguezJohn Colyer
Oct 31, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Anastasios P SaliarisJoshua M Hare
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Oct 20, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cecilia GrundtmanHåkan Westerblad
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