Regulation of calbindin-D9k expression by 1,25-dihydroxyvitamin D(3) and parathyroid hormone in mouse primary renal tubular cells

Archives of Biochemistry and Biophysics
Li-Ping CaoYan Chun Li

Abstract

Calbindin (CaBP)-D9k is a major vitamin D target gene involved in calcium homeostasis. However, studies on the molecular mechanisms of CaBP-D9k gene regulation have been hampered by the lack of an appropriate cell culture system. In the present study, we used mouse primary renal tubular cell (PRTC) cultures to investigate the regulation of CaBP-D9k expression by 1,25(OH)(2)D(3). Both CaBP-D9k mRNA and protein were highly induced by 1,25(OH)(2)D(3) in a time- and dose-dependent manner in PRTCs, and new RNA and protein synthesis was required for the induction. Transfection of VDR(-/-) cells derived from VDR null mice with human VDR restored the induction of CaBP-D9k expression by 1,25(OH)(2)D(3), confirming the requirement of VDR for CaBP-D9k expression. Treatment of the PRTCs with 1,25(OH)(2)D(3) also increased VDR protein abundance, suggesting that enhanced VDR transactivation is involved in the CaBP-D9k up-regulation. Moreover, PTH had a synergistic effect on the 1,25(OH)(2)D(3) induction of CaBP-D9k. These data demonstrate that CaBP-D9k is highly regulated by 1,25(OH)(2)D(3) and PTH in mouse PRTCs, which provides a suitable in vitro system for further investigating the molecular mechanisms involved in CaBP-D9k gene regulation.

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Citations

Oct 15, 2010·Cold Spring Harbor Perspectives in Biology·Beat Schwaller
Apr 10, 2010·Journal of the American Society of Nephrology : JASN·Yan ZhangYan Chun Li
Dec 23, 2008·Biochemical and Biophysical Research Communications·Sang-Hwan KoEui-Bae Jeung
Jul 25, 2006·International Review of Cytology·Julie Lafond, Lucie Simoneau
Jan 3, 2007·Prostate Cancer and Prostatic Diseases·Q WeiY Li
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May 29, 2009·American Journal of Physiology. Renal Physiology·Geun-Shik LeeEui-Bae Jeung
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May 10, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Guilin QiaoYan Chun Li
Mar 20, 2012·Archives of Biochemistry and Biophysics·Rajiv KumarJames R Thompson

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