Regulation of calcium binding proteins calreticulin and calsequestrin during differentiation in the myogenic cell line L6

Journal of Cellular Physiology
S TharinM Opas

Abstract

In this report we defined the structural and temporal limits within which calreticulin and calsequestrin participate in the muscle cell phenotype, in the L6 model myogenic system. Calreticulin and calsequestrin are two Ca2+ binding proteins thought to participate in intracellular Ca2+ homeostasis. We show that calsequestrin protein and mRNA were expressed when L6 cells were induced to differentiate, during which time the level of expression of calreticulin protein did not change appreciably. Calreticulin mRNA levels, however, were constant throughout L6 cell differentiation except for slight decline in the mRNA levels at the very late stages of L6 differentiation (day 11-12). We also show that the two Ca2+ binding proteins are coexpressed in differentiated L6 cells. Based on its mobility in SDS-PAGE, L6 rat skeletal muscle cells in culture expressed cardiac isoform of calsequestrin. In the mature rat skeletal muscle, calreticulin and calsequestrin were localized to sarcoplasmic reticulum (SR). Calreticulun, but not calsequestrin, staining was also observed in the perinuclear region. These data suggest that expression of calreticulin and calsequestrin may be under different control during myogenesis in rat L6 cells in culture.

References

Apr 21, 1975·Biochimica Et Biophysica Acta·G Meissner
Feb 1, 1979·The Journal of Cell Biology·A O JorgensenD H MacLennan
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J A ConnollyM W Kirschner
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M Opas, M Michalak
Mar 1, 1992·The American Journal of Physiology·M AraiM Periasamy
Aug 1, 1992·The Biochemical Journal·M MichalakM Opas
May 13, 1992·Molecular and Cellular Biochemistry·R E MilnerM Michalak
Nov 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G L Koch
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·S Fleischer, M Inui
Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Oct 1, 1986·The Journal of Cell Biology·R M KawamotoA H Caswell
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe
Apr 1, 1969·The Journal of Cell Biology·R Bischoff, H Holtzer
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J B WeinbergM A Misukonis
Jun 15, 1994·Molecular and Cellular Biochemistry·P D NashM Michalak
Jan 1, 1994·Annual Review of Physiology·C Franzini-Armstrong, A O Jorgensen
Jul 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P A HamelB L Gallie

❮ Previous
Next ❯

Citations

Dec 13, 2000·Journal of Molecular and Cellular Cardiology·B G Allen, S Katz
Nov 24, 1999·The Biochemical Journal·M MichalakM Opas
Mar 23, 1999·The Journal of Cell Biology·N MesaeliM Michalak
May 5, 2009·Trends in Cell Biology·Marek Michalak, Michal Opas
Sep 26, 2006·The International Journal of Biochemistry & Cell Biology·Sivia Barnoy, Nechama S Kosower
Jun 9, 2018·Asian-Australasian Journal of Animal Sciences·Jeong-Woong ParkTae Sub Park
Sep 22, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Maria VillagomezMichal Opas
Jan 23, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xin ZhangJingdong Yin
Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B M FreyF J Frey
Jun 11, 1999·Biochemical and Biophysical Research Communications·S E Cala

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
S BakshM Michalak
European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
M FujiwaraR Kuwano
© 2021 Meta ULC. All rights reserved