Abstract
Growing evidence indicates that a small number of cancer cells express stem cell markers and possess stem cell-like properties that promote malignant progression. Sex-determining region Y-box2 (SOX2) is a stem cell transcription factor essential for maintaining the properties of cancer stem cell (CSC). As CSC properties have been associated with angiogenesis and vasculogenic mimicry (VM), we aimed to comprehensively investigate whether SOX2 regulates CSC properties, angiogenesis, and VM in colorectal carcinoma (CRC) and its potential mechanism in this study. For this study, sphere formation assay, flow cytometry, cell survival analysis, tube formation, 3D culture, immunoblot, mouse model, and luciferase reporter assay were performed in vivo and in vitro. Expressions of SOX2 and miR-450a-5p in CRC tissue samples were examined through immunohistochemistry. First, the expression of SOX2 was not only associated with poor differentiation and prognosis but also promoted angiogenesis and VM. Knockdown of SOX2 ceased stemness properties, angiogenesis, and VM, along with decreased expression of CD133, CD31, and VE-cadherin as observed in functional experiments. Downregulation of SOX2 was found to inhibit tumorigenesis in vivo. Second, m...Continue Reading
References
Oct 7, 2004·Journal of the National Cancer Institute·Daisy W J van der SchaftMary J C Hendrix
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Apr 25, 2006·Cell·Tong Ihn LeeRichard A Young
Aug 17, 2006·Cancer Research·Shideng BaoJeremy N Rich
Feb 20, 2007·Cancer Letters·Shiwu ZhangBaocun Sun
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Piero DalerbaMichael F Clarke
Sep 21, 2007·Nature Reviews. Cancer·Richard J Gilbertson, Jeremy N Rich
Apr 1, 2008·Cancer Biology & Therapy·Jun-Yan WangBao-Cun Sun
Jun 10, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Christine E Eyler, Jeremy N Rich
Jun 2, 2009·Stem Cells and Development·Vasundhra KashyapNigel P Mongan
Jan 14, 2011·The Journal of Surgical Research·Junko MatsuokaKosei Hirakawa
Sep 20, 2011·Oncogene·U Basu-RoyC Basilico
Mar 6, 2012·Cancer Letters·Domenico Ribatti
Apr 18, 2012·Oncogene·H X ZhengB Jiang
Aug 23, 2012·PloS One·Xu HanBiaoyang Lin
Sep 15, 2012·Experimental and Therapeutic Medicine·Zhihong WengLibo Chen
Jul 23, 2013·Cancer Research·Petra M BareissClaudia Lengerke
Oct 30, 2013·Pathology Research International·Bandana SharmaShashi Chauhan
Nov 2, 2013·Oncology Letters·Yun-Long FanXin-Hua Liang
Mar 7, 2014·Cancer Research·Rebecca FavaroSilvia K Nicolis
Mar 7, 2014·The Journal of Biological Chemistry·Andrew T HaleAnne Hamik
Mar 25, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yan-Xia LuXue-Nong Li
Jun 3, 2014·Molecular Cancer·Kelong MaXiaoying Luo
Oct 30, 2014·International Journal of Molecular Sciences·Tieju LiuFang Liu
Apr 9, 2015·Therapeutic Delivery·Mitsuhiko OsakiTakahiro Ochiya
Apr 15, 2015·Oncotarget·Yuling LiLi Liang
Dec 1, 2015·Archives of Pathology & Laboratory Medicine·Jesús R GarciaPatricia Chévez-Barrios
Mar 11, 2016·Journal of Cancer Research and Clinical Oncology·Yansu ChenJianwei Zhou
Mar 8, 2017·Cancer Letters·Yue LiWangjun Liao
May 26, 2017·Molecular Carcinogenesis·Jiaxuan ChenHaoxuan Zheng
Jun 14, 2017·Cell Communication and Signaling : CCS·Bin WangLiang Zhao
Jun 23, 2018·Acta Pharmacologica Sinica·Xiao-Tian QiMei-Dan Ying
Jul 14, 2018·Journal of Experimental & Clinical Cancer Research : CR·Juan LuXiang-Ping Li
Dec 21, 2018·Nature Communications·Zhicheng ZengLi Liang
Mar 22, 2019·Cancer Research·Amit K SrivastavaQi-En Wang
Citations
Mar 19, 2020·Autophagy·Changfeng LiDaolin Tang
Sep 13, 2020·IUBMB Life·Chenchen WangMingzhu Huang
May 8, 2020·Oxidative Medicine and Cellular Longevity·Shan ShaoJianjun Lei
Jan 20, 2021·Pharmacology & Therapeutics·Lucas TrepsNicolas Clere
Feb 3, 2021·Clinical and Experimental Pharmacology & Physiology·Luming JinQiuju Yang
Aug 14, 2020·Vascular Pharmacology·Soudeh Ghafouri-FardMohammad Taheri
May 7, 2021·Cell Death & Disease·Yin ZhuHaoxuan Zheng
Sep 30, 2021·Expert Reviews in Molecular Medicine·Sepideh MirzaeiHaroon Khan