Regulation of Candida albicans Interaction with Macrophages through the Activation of HOG Pathway by Genistein

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Shuna CuiUrsula Bilitewski

Abstract

The severity of infections caused by Candida albicans, the most common opportunistic human fungal pathogen, needs rapid and effective antifungal treatments. One of the effective ways is to control the virulence factors of the pathogen. Therefore, the current study examined the effects of genistein, a natural isoflavone present in soybeans, on C. albicans. The genistein-treated C. albicans cells were then exposed to macrophages. Although no inhibition effect on the growth rates of C. albicans was noted an enhancement of the immune response to macrophages has been observed, indicated by phagocytosis and release of cytokines TNF-α and IL-10. The effect of genistein on the enhanced phagocytosis can be mimicked by the fungicides fludioxonil or iprodione, which inhibit the histidine kinase Cos1p and lead to activation of HOG pathway. The western blot results showed a clear phosphorylation of Hog1p in the wild type strain of C. albicans after incubation with genistein. In addition, effects of genistein on the phosphorylation of Hog1p in the histidine kinase mutants Δcos1 and Δsln1 were also observed. Our results thus indicate a new bio-activity of genistein on C. albicans by activation of the HOG pathway of the human pathogen C. albic...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·T Akiyama, H Ogawara
Apr 1, 1986·The Journal of Antibiotics·H OgawaraK Suzuki
Aug 31, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A Casadevall, L A Pirofski
Oct 2, 2003·Bioscience, Biotechnology, and Biochemistry·Chunyeon ChoiYoungsun Song
Nov 12, 2005·Science·Rachael Z MurrayJennifer L Stow
Feb 21, 2006·FEMS Yeast Research·Michael Kruppa, Richard Calderone
Nov 17, 2007·The Journal of Infectious Diseases·Neil A R GowBart Jan Kullberg
Sep 4, 2008·Infection and Immunity·Neeraj Chauhan, Richard Calderone
Aug 4, 2009·Journal of Applied Microbiology·J WesolowskiU Bilitewski
Aug 25, 2012·Journal of Medical Microbiology·Anna BuschartUrsula Bilitewski
Sep 7, 2013·Biomolecules & Therapeutics·Sainkhuu BatbayarHa Won Kim
Dec 3, 2013·International Immunopharmacology·Shuna CuiUrsula Bilitewski

❮ Previous
Next ❯

Citations

Sep 21, 2016·International Journal of Molecular Sciences·Seockmo KuHyun Ju You
Nov 17, 2019·Journal of Fungi·Verónica UrrialdeRebeca Alonso-Monge
Apr 4, 2021·Sensors·Zsigmond PappGábor Papp
Jul 3, 2021·Journal of Fungi·Che-Kang ChangChung-Yu Lan

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
ELISA

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.