Regulation of carbohydrate transport activities in Salmonella typhimurium: use of the phosphoglycerate transport system to energize solute uptake.

Journal of Bacteriology
M H Saier, B U Feucht

Abstract

The phosphoglycerate transport system was employed to supply energy-depleted, lysozyme-treated Salmonella typhimurium cells with a continuous intracellular source of phosphoenolpyruvate. When the cells had been induced to high levels of the phosphoglycerate transport system, a low extracellular concentration of phosphoenolpyruvate (0.1 mM) half maximally stimulated uptake of methyl alpha-glucoside via the phosphoenolpyruvate:sugar phosphotransferase system. If the phosphoglycerate transport system was not induced before energy depletion, 100 times this concentration of phosphoenolpyruvate was required for half-maximal stimulation. Phosphoenolpyruvate could not be replaced by other energy sources if potassium fluoride (an inhibitor of enolase) was present. Inhibition of [14C]-glycerol uptake into energy-depleted cells by methyl alpha-glucoside was demonstrated. A concentration of phosphoenolpyruvate which stimulated methyl alpha-glucoside accumulation counteracted the inhibitory effect of the glucoside. In the presence of potassium fluoride, phosphoenolpyruvate could not be replaced by other energy sources. Inhibition of glycerol uptake by methyl alpha-glucoside in intact untreated cells was also counteracted by phosphoenolpyruv...Continue Reading

References

Dec 14, 1976·Biochimica Et Biophysica Acta·P W Postma, S Roseman
Jul 26, 1968·Biochemical and Biophysical Research Communications·Y SannoE C Lin
Jan 1, 1970·Annual Review of Genetics·E C Lin

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Citations

Mar 11, 2020·Microbial Biotechnology·Antoine Danchin
Dec 1, 1981·Microbiological Reviews·J L Botsford
Jul 1, 1984·Journal of Bacteriology·P H Reibach, J G Streeter
Sep 1, 1980·Microbiological Reviews·S S DillsM H Saier

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