Regulation of cardiac IKs potassium current by membrane phosphatidylinositol 4,5-bisphosphate

The Journal of Biological Chemistry
Wei-Guang DingHiroshi Matsuura

Abstract

Regulation of the slowly activating component of delayed rectifier K+ current (IKs) by membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PtdIns-(4,5)P2) was examined in guinea pig atrial myocytes using the whole-cell patch clamp method. IKs was elicited by depolarizing voltage steps given from a holding potential of -50 mV, and the effect of various test reagents on IKs was assessed by measuring the amplitude of tail current elicited upon return to the holding potential following a 2-s depolarization to +30 mV. Intracellular application of 50 microM wortmannin through a recording pipette evoked a progressive increase in IKs over a 10-15-min period to 208.5 +/- 14.6% (n = 9) of initial magnitude obtained shortly after rupture of the patch membrane. Intracellular application of anti-PtdIns(4,5)P2 monoclonal antibody also increased the amplitude of IKs to 198.4 +/- 19.9% (n = 5). In contrast, intracellular loading with exogenous PtdIns(4,5)P2 at 10 and 100 mum produced a marked decrease in the amplitude of IKs to 54.3 +/- 3.8% (n = 5) and 44.8 +/- 8.2% (n = 5), respectively. Intracellular application of neomycin (50 microM) or aluminum (50 microM) evoked an increase in the amplitude of IKs to 161.0 +/- 13.5% (n = 4) and...Continue Reading

References

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Citations

Feb 15, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Laszlo KölesPeter Illes
May 31, 2007·Pflügers Archiv : European journal of physiology·Donald W Hilgemann
Dec 22, 2007·Pflügers Archiv : European journal of physiology·Sergey MissanTerence F McDonald
May 1, 2009·Pflügers Archiv : European journal of physiology·Dimitar P ZankovMinoru Horie
Jun 1, 2005·Current Opinion in Neurobiology·Byung-Chang Suh, Bertil Hille
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yang LiJianmin Cui
Feb 3, 2006·American Journal of Physiology. Cell Physiology·Wen-Zhi LanCeredwyn E Hill
Oct 19, 2010·Journal of Molecular and Cellular Cardiology·Wei-Guang DingHiroshi Matsuura

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