Regulation of cardiotrophin-1 expression in mouse embryonic stem cells by HIF-1alpha and intracellular reactive oxygen species

Journal of Cell Science
Bernadette AteghangHeinrich Sauer

Abstract

Cardiomyogenesis in differentiating mouse embryonic stem (ES) cells is promoted by cardiotrophin-1 (CT-1), a member of the IL-6 interleukin superfamily that acts through the tall gp130 cytokine receptor. We show that prooxidants (menadione, hydrogen peroxide) as well as chemical (CoCl2) and physiological (1% O2) hypoxia increased CT-1 as well as HIF-1alpha protein and mRNA expression in embryoid bodies, indicating that CT-1 expression is regulated by reactive oxygen species (ROS) and hypoxia. Treatment with either prooxidants or chemical hypoxia increased gp130 phosphorylation and protein expression of NADPH oxidase subunits p22-phox, p47-phox, p67-phox, as well as Nox1 and Nox4 mRNA. Consequently, inhibition of NADPH oxidase activity by diphenylen iodonium chloride (DPI) and apocynin abolished prooxidant- and chemical hypoxia-induced upregulation of CT-1. Prooxidants and chemical hypoxia activated ERK1,2, JNK and p38 as well as PI3-kinase. The proxidant- and CoCl2-mediated upregulation of CT-1 was significantly inhibited in the presence of the ERK1,2 antagonist UO126, the JNK antagonist SP600125, the p38 antagonist SKF86002, the PI3-kinase antagonist LY294002, the Jak-2 antagonist AG490 as well as in the presence of free radic...Continue Reading

References

Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·M GassmannH Acker
Nov 1, 1996·American Journal of Respiratory Cell and Molecular Biology·C MarshallB E Marshall
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·N S ChandelP T Schumacker
Feb 11, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S BichetM Gassmann
Oct 26, 1999·Biochemical and Biophysical Research Communications·S HishinumaK Yamauchi-Takihara
Nov 26, 1999·Journal of Cellular Biochemistry·H SauerM Wartenberg
Jun 22, 2000·Journal of Molecular and Cellular Cardiology·M FunamotoK Yamauchi-Takihara
Apr 24, 2002·American Journal of Respiratory Cell and Molecular Biology·Ioanna KosmidouCharis Roussos
Aug 30, 2002·Biochemical and Biophysical Research Communications·John J Haddad
May 3, 2003·Cancer Cell·Nicola J MabjeeshParaskevi Giannakakou
May 30, 2003·The Biochemical Journal·Peter C HeinrichFred Schaper
Aug 26, 2003·FEBS Letters·Audrey CarrièreLouis Casteilla
Dec 17, 2003·Molecular and Cellular Biochemistry·Darren H FreedIan M C Dixon
Mar 26, 2004·Methods in Molecular Biology·Gisele HöpflIsabelle Desbaillets
Oct 12, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hiroshi KidaIchiro Kawase
Feb 3, 2005·International Immunopharmacology·John J Haddad, Hisham L Harb
Feb 22, 2005·Cardiovascular Research·Darren H FreedIan M C Dixon
Mar 25, 2005·American Journal of Physiology. Cell Physiology·Li Zuo, Thomas L Clanton
Jul 9, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael S WolinSachin A Gupte
Aug 2, 2005·Cell Metabolism·Joslyn K BrunelleNavdeep S Chandel

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Citations

Nov 13, 2008·Antioxidants & Redox Signaling·Marcia A Ogasawara, Hui Zhang
Mar 25, 2009·Antioxidants & Redox Signaling·Masuko Ushio-Fukai, Norifumi Urao
Feb 19, 2010·Experimental & Molecular Medicine·Ae-Ri JiYoung Min Choi
Sep 17, 2008·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·David Stejskal, Viktor Ruzicka
Oct 17, 2012·Antioxidants & Redox Signaling·Pooja ChaudhariYoon-Young Jang
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Beek Y ChinLeo E Otterbein
May 22, 2013·Mediators of Inflammation·Mohamed AsrihFabrizio Montecucco
Dec 7, 2014·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Shu-Fen ChenZhan-Ling Dong
May 8, 2015·Journal of Drug Targeting·Shuhui Guo, Xiuping Chen
Feb 22, 2008·Thrombosis Research·Pantila VanichakarnSubrata Chakrabarti
Aug 25, 2007·European Journal of Cell Biology·Ingo HaaseGustav Mahrle
Jul 3, 2015·Stem Cells and Development·Anaïs WanetPatricia Renard
Aug 3, 2014·The Journal of Surgical Research·Carmen HerenciaJuan Rafael Muñoz-Castañeda
Feb 2, 2010·Journal of Molecular and Cellular Cardiology·Kwong-Man NgHung-Fat Tse
Aug 15, 2015·Oxidative Medicine and Cellular Longevity·Tullia MaraldiAnto De Pol
Jun 16, 2007·Cellular Signalling·Marcelo Genestra
May 26, 2015·International Journal of Cardiology·Lena MascheckHeinrich Sauer
May 17, 2011·Free Radical Biology & Medicine·Caroline BartschMaria Wartenberg
Jun 25, 2013·The American Journal of Pathology·Sibylle SabrautzkiMartin Hrabě de Angelis
Oct 26, 2016·Journal of Pineal Research·Jana KudováLukáš Kubala
Sep 26, 2009·Current Opinion in Organ Transplantation·Jeffrey R MillmanClark K Colton
Apr 19, 2015·Journal of Translational Medicine·Xiaotian SunYiqing Wang
Oct 21, 2016·Functional & Integrative Genomics·Jun FanYunguang Tong
Aug 19, 2020·The Journal of Clinical Investigation·Jenny E Gunton
Apr 3, 2020·Scientific Reports·Kapka MitevaKarim J Brandt
Jun 26, 2021·World Journal of Diabetes·Marko KumricJosko Bozic

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