PMID: 9556913Apr 29, 1998Paper

Regulation of CAT protein by ribozyme and antisense mRNA in transgenic mice

Transgenic Research
D L SokolJ D Murray

Abstract

Transgenic mouse lines were engineered to express stably antisense mRNA or antisense mRNA containing catalytic ribozyme (rbz) structures complementary to bacterial chloramphenicol acetyltransferase (CAT) gene transcripts. One transgenic line expressed antisense mRNA that specifically targeted full-length CAT coding sequences (ACAT). Another transgenic line expressed full-length antisense CAT mRNA which was modified by mutagensis to include four rbz cassettes (rbz-ACAT) in order to compare antisense versus antisense-rbz function in vivo. Preliminary data were also collected from a transgenic mouse line expressing antisense mRNA targeting 72% of the 5' region of CAT coding sequences (5' ACAT). All constructs contained similar control elements in their design. Promoter elements were derived from the bovine alpha s1-casein gene, while the small t intron and 3' control sequences were derived from SV40. The ability of these various constructs to down-regulate CAT protein levels was compared by analysis of CAT protein production in lactating double-hemizygous transgenic female mice. Every double-hemizygous mouse analysed expressed mRNA from the alpha s1-casein-CAT construct (Clarke et al., 1994) and equivalent levels of mRNA from one ...Continue Reading

Citations

Nov 25, 1998·Nucleic Acids Research·N K VaishF Eckstein
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·R P Erickson
Nov 20, 1998·Current Opinion in Biotechnology·P J WelchF Wong-Staal
Mar 23, 2000·Theriogenology·J D Murray

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