Regulation of catalase activity and gene expression during Phytophthora nicotianae development and infection of tobacco.

Molecular Plant Pathology
Leila M Blackman, A R Hardham

Abstract

Plant defence against pathogen attack typically incorporates an oxidative burst involving elevated levels of reactive oxygen species such as hydrogen peroxide. In the present study, we have used an in-gel assay to monitor the activity of the hydrogen peroxide scavenging enzyme, catalase, during asexual development of Phytophthora nicotianae and during infection of host tobacco plants. In vitro, catalase activity is highest in sporulating hyphae; in planta, catalase activity increases dramatically about 8 h after host inoculation. We have cloned and characterized three catalase genes, designated PnCat1, PnCat2 and PnCat3, from P. nicotianae and identified their homologues in P. infestans, P. sojae and P. ramorum. In all three species, Cat2 is predicted to be targeted to the peroxisome and the other catalases are likely to be cytosolic. Quantitative real-time PCR assessment of catalase transcripts during development and infection indicates that peroxisomal PnCat2 is the gene predominantly expressed, with transcript levels peaking in vitro in sporulating hyphae and in planta increasing dramatically during the first 24 h after inoculation of susceptible tobacco seedlings. Levels of tobacco catalase gene expression are significantly...Continue Reading

References

Aug 1, 1992·Applied and Environmental Microbiology·M G Klotz, S W Hutcheson
Jul 1, 1992·Archives of Biochemistry and Biophysics·E LevyA Hochman
Jan 1, 1990·Methods in Enzymology·C R Merril
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R W Skadsen, J G Scandalios
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J H GundersonM L Sogin
Jun 23, 1995·Journal of Molecular Biology·P GouetO Dideberg
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Z ChenD F Klessig
Aug 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M Kozak
Jan 1, 1996·Free Radical Biology & Medicine·J A EscobarE A Lissi
Sep 1, 1996·Plant Physiology·J A FrugoliC R McClung
Apr 25, 1997·Journal of Molecular Biology·C Burge, S Karlin
May 1, 1997·The Plant Journal : for Cell and Molecular Biology·H TakahashiD F Klessig
Aug 1, 1997·The Plant Journal : for Cell and Molecular Biology·R T MullenR N Trelease
Nov 14, 1997·Infection and Immunity·J A CaleraJ P Latgé
May 14, 1999·Trends in Plant Science·S KamounV G Vleeshouwers
Jan 29, 2000·Molecular Microbiology·X Q Xu, S Q Pan
Jun 2, 2001·Molecular Plant-microbe Interactions : MPMI·R SantosD Expert
Dec 14, 2001·Protein Science : a Publication of the Protein Society·Koodathingal PrakashVinod Bhakuni
Feb 16, 2002·Bioinformatics·Hideo BannaiSatoru Miyano
May 9, 2002·Journal of Experimental Botany·Eva VranováFrank Van Breusegem
May 9, 2002·Journal of Experimental Botany·Luis A del RíoJuan B Barroso
May 9, 2002·Journal of Experimental Botany·Ruth Grene AlscherLenwood S Heath
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tanja Petnicki-OcwiejaJames R Alfano
May 30, 2002·Biological Chemistry·Leonardo Peraza, Wilhelm Hansberg
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Guido FellbrichThorsten Nürnberger

❮ Previous
Next ❯

Citations

Oct 23, 2012·Protoplasma·Jerónimo Guzmán-DearaErnesto García-Pineda
May 11, 2010·Fungal Genetics and Biology : FG & B·Reena D NarayanAdrienne R Hardham
Aug 16, 2012·Environmental Microbiology·Zheng-Liang WangMing-Guang Feng
Jan 10, 2013·Plant Biotechnology Journal·Angelo KaldisRima Menassa
Dec 15, 2010·International Journal for Parasitology·Géraldine DubreuilMarie-Noëlle Rosso
Jul 29, 2010·Protist·Leila M BlackmanAdrienne R Hardham
May 19, 2017·Molecular Plant Pathology·Adrienne R Hardham, Leila M Blackman
Mar 17, 2020·Frontiers in Plant Science·Huai-Xia ZhangZhen-Hui Gong
Oct 18, 2020·Molecular Biology Reports·Darling de Andrade LourençoAltino Choupina
Apr 2, 2021·Plant Physiology·Jade R Bleau, Steven H Spoel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved