Regulation of cathepsin D dependent on the phenotype of colon carcinoma cells

International Journal of Cancer. Journal International Du Cancer
S Hennebicq ReigG Huet

Abstract

We have studied the intracellular trafficking of cathepsin D in different colon carcinoma cell populations: the HT-29 cell line, composed of >95% undifferentiated cells; 2 subpopulations derived from this cell line, containing cells committed to differentiation into mucin-secreting cells (HT-29 MTX) or enterocyte-like cells (HT-29 G-) after confluence; and the Caco-2 cell line, which spontaneously differentiates into enterocyte-like cells after confluence. Post-confluent undifferentiated HT-29 cells and differentiated enterocyte-like HT-29 G- and Caco-2 cells secrete significant levels of cathepsin D in culture medium, in contrast to post-confluent differentiated mucin-secreting HT-29 MTX cells, which secrete this enzyme at a very low level. The intracellular content and the mRNA level of cathepsin D increase after confluence in the different cell types, particularly in Caco-2 cells, which intensify the secretion of cathepsin D along with the differentiation process post-confluence. Membrane-associated mature cathepsin D was detected in HT-29 cells but not in Caco-2 cells. In the different types of cell, pro-cathepsin D associates with the membrane concomitantly to its binding to an Mr 72,000 protein. Membrane association persi...Continue Reading

References

Jul 1, 1992·The American Journal of Physiology·B HimpensR Casteels
Nov 11, 1991·International Journal of Cancer. Journal International Du Cancer·T LesuffleurA Zweibaum
Dec 1, 1990·Cancer Metastasis Reviews·H RochefortM Garcia
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D L Helseth, A Veis
Jul 1, 1983·The Journal of Cell Biology·V GieselmannK Von Figura
Jun 15, 1994·International Journal of Cancer. Journal International Du Cancer·G HuetP Degand

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Citations

Mar 25, 2000·Diseases of the Colon and Rectum·J AraoT Fujimori
Jun 8, 2001·European Journal of Biochemistry·O J CorderoF J Rodríguez-Berrocal

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