Regulation of CCK-induced amylase release by PKC-delta in rat pancreatic acinar cells

American Journal of Physiology. Gastrointestinal and Liver Physiology
Chenwei LiJohn A Williams

Abstract

PKC is known to be activated by pancreatic secretagogues such as CCK and carbachol and to participate along with calcium in amylase release. Four PKC isoforms, alpha, delta, epsilon, and zeta, have been identified in acinar cells, but which isoforms participate in amylase release are unknown. To identify the responsible isoforms, we used translocation assays, chemical inhibitors, and overexpression of individual isoforms and their dominant-negative variants by means of adenoviral vectors. CCK stimulation caused translocation of PKC-alpha, -delta, and -epsilon, but not -zeta from soluble to membrane fraction. CCK-induced amylase release was inhibited approximately 30% by GF109203X, a broad spectrum PKC inhibitor, and by rottlerin, a PKC-delta inhibitor, but not by Gö6976, a PKC-alpha inhibitor, at concentrations from 1 to 5 microM. Neither overexpression of wild-type or dominant-negative PKC-alpha affected CCK-induced amylase release. Overexpression of PKC-delta and -epsilon enhanced amylase release, whereas only dominant-negative PKC-delta inhibited amylase release by 25%. PKC-delta overexpression increased amylase release at all concentrations of CCK, but dominant-negative PKC-delta only inhibited the maximal concentration; bo...Continue Reading

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Citations

Jul 14, 2010·Current Opinion in Gastroenterology·John A Williams
Jul 23, 2011·Current Opinion in Gastroenterology·Rashmi Chandra, Rodger A Liddle
Apr 11, 2008·Journal of Gastroenterology and Hepatology·Fred GorelickEdwin Thrower
Oct 1, 2011·American Journal of Physiology. Cell Physiology·Hatim A HassanPeter S Aronson
Apr 5, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Edwin C ThrowerFred S Gorelick
Sep 18, 2010·The Journal of Veterinary Medical Science·Kazumi TaniguchiKen-ichiro Mutoh
Jun 19, 2013·PloS One·Maria Eugenia Sabbatini, John A Williams
Oct 24, 2012·BMC Biotechnology·Isabelle HoubrackenLuc Bouwens
Feb 20, 2007·Biochimica Et Biophysica Acta·Marc J BernaRobert T Jensen
Oct 27, 2017·International Journal of Molecular Sciences·Yoo Kyung Jeong, Hyeyoung Kim
Aug 8, 2006·Current Opinion in Gastroenterology·John A Williams
Apr 1, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Akihiko SatohStephen J Pandol
Jul 4, 2006·Physiological Reviews·Marlène DufresneDaniel Fourmy
Jul 19, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Vandanajay BhatiaMiriam Falzon
Nov 27, 2009·American Journal of Physiology. Cell Physiology·Maria E SabbatiniJohn A Williams
Jan 9, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Keitaro SatohYoshiteru Seo
Nov 5, 2019·Scientific Reports·Jeetindra R A BalakFrançoise Carlotti
Jun 13, 2009·Alcoholism, Clinical and Experimental Research·Andreas GerloffPeter Feick
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Jun 16, 2011·Neurochemistry International·Eun-Joo ShinHyoung-Chun Kim

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