PMID: 11312258Apr 20, 2001Paper

Regulation of CD21 expression by DNA methylation and histone deacetylation

International Immunology
J Schwab, Harald Illges

Abstract

The complement receptor II (CD21) serves as a receptor for the complement component C3d of immune complexes on B lymphocytes. Expression of the CD21 gene is tightly regulated during B lymphocyte differentiation. Only mature B lymphocytes, but not pro-, pre- or plasma B lymphocytes, express CD21. There is evidence that cell type-specific expression is mediated by a silencer element located in the first intron. The CD21 promoter region contains a CpG island adjacent to the ATG start codon. We have analyzed the methylation status of this CpG island in B lymphoid cell lines representing the various differentiation stages of B lymphocyte development and primary lymphocytes. We found that the pro-, pre- and intermediate B lymphocytes contain a methylated CpG island and do not express CD21, whereas CD21-expressing mature B lymphocytes, plasma B lymphocytes and non-lymphoid cells carry a demethylated CD21 CpG island. To analyze whether the lack of CD21 expression in early B lymphocytes is due to inhibition by CpG methylation we have used 5-aza-2'-deoxycytidine to inhibit DNA methyltransferase activity. Treatment of pro-B lymphocytes with the drug resulted in expression of CD21. We have also applied Trichostatin A (TSA), an inhibitor of...Continue Reading

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Citations

Jun 17, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ildikó Györy, Janos Minarovits
Dec 3, 2014·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Hildegard T GreinixWinfried F Pickl
Jul 25, 2012·Trends in Genetics : TIG·Beatriz Suarez-AlvarezCarlos López-Larrea
Nov 13, 2007·Molecular Immunology·Satoru FujimuraNobuo Sakaguchi
Aug 3, 2007·Biochimica Et Biophysica Acta·Yann CheliThomas J Kunicki
Dec 12, 2001·The Journal of Biological Chemistry·Ana Lucia B CabralVilma R Martins
Apr 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Soizic GaraudYves Renaudineau
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irina DebnathJohn H Weis
Mar 20, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shin-ichi HashimotoKouji Matsushima

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