Regulation of cell cycle and stress responses under nitrosative stress in Schizosaccharomyces pombe

Free Radical Biology & Medicine
Uddalak MajumdarSanjay Ghosh

Abstract

Nitric oxide (NO) acts as a signaling molecule in numerous physiological processes but excess production generates nitrosative stress in cells. The exact protective mechanism used by cells to combat nitrosative stress is unclear. In this study, the fission yeast Schizosaccharomyces pombe has been used as a model system to explore cell cycle regulation and stress responses under nitrosative stress. Exposure to an NO donor results in mitotic delay in cells through G2/M checkpoint activation and initiates rereplication. Western blot analysis of phosphorylated Cdc2 revealed that the G2/M block in the cell cycle was due to retention of its inactive phosphorylated form. Interestingly, nitrosative stress results in inactivation of Cdc25 through S-nitrosylation that actually leads to cell cycle delay. From differential display analysis, we identified plo1, spn4, and rga5, three cell cycle-related genes found to be differentially expressed under nitrosative stress. Exposure to nitrosative stress also results in abnormal septation and cytokinesis in S. pombe. In summary we propose a novel molecular mechanism of cell cycle control under nitrosative stress based on our experimental results and bioinformatics analysis.

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Citations

Apr 30, 2015·Journal of Cellular Biochemistry·Armance GelaudeJean-François Bodart
Dec 10, 2015·Nitric Oxide : Biology and Chemistry·Rika Indri AstutiHiroshi Takagi
Aug 12, 2014·Biochemical and Biophysical Research Communications·Chiranjit Panja, Sanjay Ghosh
Sep 1, 2016·Oxidative Medicine and Cellular Longevity·Jan NevoralJaroslav Petr
Oct 21, 2016·Applied Microbiology and Biotechnology·Rika Indri AstutiHiroshi Takagi
Jan 23, 2020·Cells·Armance GelaudeJean-François Bodart
Jul 23, 2014·Scandinavian Journal of Gastroenterology·Takahiro ZendaHidekuni Inadera
Jun 26, 2020·Frontiers in Microbiology·Marina V NavarroWagner L Batista
Jan 13, 2021·Plant Physiology and Biochemistry : PPB·Mona F A DawoodArafat Abdel Hamed Abdel Latef

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