Regulation of cell growth by IRF-1 in BHK-21 cells.

Cytotechnology
S KirchhoffH Hauser

Abstract

Most cell lines that are used for the production of recombinant proteins proliferate spontaneously at a high rate. In many types of cultivation systems these cells still keep growing after having reached the desired cell density. Further proliferation in batch cultures leads to cell death as a consequence of nutrient and oxygen depletion as well as to accumulation of lactate and toxic products. Consequently, in many technical processes, the surplus of cells is removed.We have established cell lines in which proliferation is controlled by a physiological regulator, IRF-1. IRF-1 (Interferon Regulatory Factor 1) is a transcriptional activator and acts as a tumor suppressor. Constitutive overexpression of recombinant IRF-1 leads to inhibition of cell growth. The extent of this growth arrest depends on the intracellular concentration of active IRF-1. To allow IRF-1 expression in various mammalian cells a system for conditional IRF-1 activation has been established. A fusion protein composed of IRF-1 and the hormone binding domain of the human estrogen receptor, was used. This system allows to control gradually the growth of several mammalian cell lines by adjusting the intracellular concentration of active IRF-1 via estradiol in the...Continue Reading

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Citations

Oct 12, 1999·Biotechnology and Bioengineering·P P MuellerH Hauser
Dec 18, 2001·Biotechnology and Bioengineering·S SchlatterM Fussenegger
Nov 13, 2008·Cytotechnology·Darrin KuystermansMohamed Al-Rubeai
Oct 18, 2001·Journal of Biotechnology·A V CarvalhalM J Carrondo
Feb 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Andrea KrögerPeter P Mueller
Aug 7, 2008·Biotechnology and Bioengineering·Soo Hean Gary Khoo, Mohamed Al-Rubeai
Apr 16, 2010·Biotechnology and Applied Biochemistry·Yiping LimMiranda G S Yap
Jun 22, 2000·Biotechnology and Bioengineering·C GeserickP P Mueller
Mar 31, 2018·Bioscience Reports·Khaldoon Alsamman, Omar S El-Masry

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